1z8s
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | |GENE= dnaG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 Geobacillus stearothermophilus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z8s OCA], [http://www.ebi.ac.uk/pdbsum/1z8s PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z8s RCSB]</span> | ||
}} | }} | ||
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[[Category: two alpha helical sub-domain]] | [[Category: two alpha helical sub-domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:32:11 2008'' |
Revision as of 22:32, 30 March 2008
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| Gene: | dnaG (Geobacillus stearothermophilus) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)
Overview
The helicase-primase interaction is a critical event in DNA replication and is mediated by a putative helicase-interaction domain within the primase. The solution structure of the helicase-interaction domain of DnaG reveals that it is made up of two independent subdomains: an N-terminal six-helix module and a C-terminal two-helix module that contains the residues of the primase previously identified as important in the interaction with the helicase. We show that the two-helix module alone is sufficient for strong binding between the primase and the helicase but fails to activate the helicase; both subdomains are required for helicase activation. The six-helix module of the primase has only one close structural homolog, the N-terminal domain of the corresponding helicase. This surprising structural relationship, coupled with the differences in surface properties of the two molecules, suggests how the helicase-interaction domain may perturb the structure of the helicase and lead to activation.
About this Structure
1Z8S is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation., Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP, Structure. 2005 Apr;13(4):609-16. PMID:15837199
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