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3egz

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Revision as of 09:11, 25 October 2017

Crystal structure of an in vitro evolved tetracycline aptamer and artificial riboswitch

Structural highlights

3egz is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Gene:	SNRPA (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The tetracycline aptamer is an in vitro selected RNA that binds to the antibiotic with the highest known affinity of an artificial RNA for a small molecule (Kd approximately 0.8 nM). It is one of few aptamers known to be capable of modulating gene expression in vivo. The 2.2 A resolution cocrystal structure of the aptamer reveals a pseudoknot-like fold formed by tertiary interactions between an 11 nucleotide loop and the minor groove of an irregular helix. Tetracycline binds within this interface as a magnesium ion chelate. The structure, together with previous biochemical and biophysical data, indicates that the aptamer undergoes localized folding concomitant with tetracycline binding. The three-helix junction, h-shaped architecture of this artificial RNA is more complex than those of most aptamers and is reminiscent of the structures of some natural riboswitches.

Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch.,Xiao H, Edwards TE, Ferre-D'Amare AR Chem Biol. 2008 Oct 20;15(10):1125-37. PMID:18940672^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lutz CS, Cooke C, O'Connor JP, Kobayashi R, Alwine JC. The snRNP-free U1A (SF-A) complex(es): identification of the largest subunit as PSF, the polypyrimidine-tract binding protein-associated splicing factor. RNA. 1998 Dec;4(12):1493-9. PMID:9848648
↑ Xiao H, Edwards TE, Ferre-D'Amare AR. Structural basis for specific, high-affinity tetracycline binding by an in vitro evolved aptamer and artificial riboswitch. Chem Biol. 2008 Oct 20;15(10):1125-37. PMID:18940672 doi:10.1016/j.chembiol.2008.09.004

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3egz"

@@ Line 1: / Line 1: @@
 ==Crystal structure of an in vitro evolved tetracycline aptamer and artificial riboswitch==
 <StructureSection load='3egz' size='340' side='right' caption='[[3egz]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SNRPA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egz OCA], [http://pdbe.org/3egz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3egz RCSB], [http://www.ebi.ac.uk/pdbsum/3egz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3egz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3egz OCA], [http://pdbe.org/3egz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3egz RCSB], [http://www.ebi.ac.uk/pdbsum/3egz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3egz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 29: / Line 30: @@
 </div>
 <div class="pdbe-citations 3egz" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Nucleoprotein|Nucleoprotein]]
 == References ==
 <references/>

3egz

From Proteopedia

Revision as of 09:11, 25 October 2017

Crystal structure of an in vitro evolved tetracycline aptamer and artificial riboswitch

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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