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3gtn

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Revision as of 07:12, 1 November 2017

Crystal Structure of XynC from Bacillus subtilis 168

Structural highlights

3gtn is a 2 chain structure with sequence from "vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	BSU18150, xynC, ynfF ("Vibrio subtilis" Ehrenberg 1835)
Activity:	Glucuronoarabinoxylan endo-1,4-beta-xylanase, with EC number 3.2.1.136
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[XYNC_BACSU] Catalyzes the depolymerization of methylglucuronoxylan (MeGAXn) from different sources. It cleaves the beta-1,4-xylosidic bond penultimate to that linking carbon one of the xylose residue substituted with alpha-1,2-linked 4-O-methyl-D-glucuronate (MeGA).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The recent biochemical characterization of the xylanases of glycosyl hydrolase family 5 (GH 5) has identified a distinctive endo mode of action, hydrolyzing the beta-1,4 xylan chain at a specific site directed by the position of an alpha-1,2-linked glucuronate moiety. Xylanase C (XynC), the GH 5 xylanase from Bacillus subtilis 168, has been cloned, overexpressed and crystallized. Initial data collection was performed and a preliminary model has been built into a low-quality 2.7 A resolution density map. The crystals belonged to the primitive monoclinic space group P2(1). Further screening identified an additive that resulted in large reproducible crystals. This larger more robust crystal form belonged to space group P2(1)2(1)2 and a resulting data set has been processed to 1.64 A resolution. This will be the second structure to be solved from this unique xylanase family and the first from a Gram-positive bacterium. This work may help to identify the structural determinants that allow the exceptional specificity of this enzyme and the role it plays in the biological depolymerization and processing of glucuronoxylan.

Crystallization and crystallographic analysis of Bacillus subtilis xylanase C.,St John FJ, Godwin DK, Preston JF, Pozharski E, Hurlbert JC Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt, 5):499-503. Epub 2009 Apr 24. PMID:19407387^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ St John FJ, Rice JD, Preston JF. Characterization of XynC from Bacillus subtilis subsp. subtilis strain 168 and analysis of its role in depolymerization of glucuronoxylan. J Bacteriol. 2006 Dec;188(24):8617-26. Epub 2006 Oct 6. PMID:17028274 doi:JB.01283-06
↑ St John FJ, Godwin DK, Preston JF, Pozharski E, Hurlbert JC. Crystallization and crystallographic analysis of Bacillus subtilis xylanase C. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt, 5):499-503. Epub 2009 Apr 24. PMID:19407387 doi:10.1107/S1744309109013098

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3gtn"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of XynC from Bacillus subtilis 168==
 <StructureSection load='3gtn' size='340' side='right' caption='[[3gtn]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3gtn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GTN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GTN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3gtn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GTN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GTN FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU18150, xynC, ynfF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU18150, xynC, ynfF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucuronoarabinoxylan_endo-1,4-beta-xylanase Glucuronoarabinoxylan endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.136 3.2.1.136] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gtn OCA], [http://pdbe.org/3gtn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gtn RCSB], [http://www.ebi.ac.uk/pdbsum/3gtn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gtn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gtn OCA], [http://pdbe.org/3gtn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gtn RCSB], [http://www.ebi.ac.uk/pdbsum/3gtn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gtn ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus globigii migula 1900]]
+[[Category: Vibrio subtilis ehrenberg 1835]]
 [[Category: Glucuronoarabinoxylan endo-1,4-beta-xylanase]]
 [[Category: Hurlbert, J C]]

3gtn

From Proteopedia

Revision as of 07:12, 1 November 2017

Crystal Structure of XynC from Bacillus subtilis 168

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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