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3ks8

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Revision as of 08:40, 1 November 2017

Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA

Structural highlights

3ks8 is a 6 chain structure with sequence from Ebore. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3ks4, 3fke
Gene:	REBOVgp2, VP35 (EBORE)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VP35_EBORR] Acts as a polymerase cofactor in the RNA polymerase transcription and replication complex. Prevents establishment of cellular antiviral state by blocking virus-induced phosphorylation and activation of interferon regulatory factor 3 (IRF3), a transcription factor critical for the induction of interferons alpha and beta. The mechanism by which this blockage occurs remains incompletely defined, a hypothesis suggests that VP35 dsRNA-binding activity prevents activation of IRF3 by sequestering dsRNA. Also inhibits the antiviral effect mediated by the interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Ebolavirus causes a severe hemorrhagic fever and is divided into five distinct species, of which Reston ebolavirus is uniquely nonpathogenic to humans. Disease caused by ebolavirus is marked by early immunosuppression of innate immune signaling events, involving silencing and sequestration of double-stranded RNA (dsRNA) by the viral protein VP35. Here we present unbound and dsRNA-bound crystal structures of the dsRNA-binding domain of Reston ebolavirus VP35. The structures show that VP35 forms an unusual, asymmetric dimer on dsRNA binding, with each of the monomers binding dsRNA in a different way: one binds the backbone whereas the other caps the terminus. Additional SAXS, DXMS, and dsRNA-binding experiments presented here support a model of cooperative dsRNA recognition in which binding of the first monomer assists binding of the next monomer of the oligomeric VP35 protein. This work illustrates how ebolavirus VP35 could mask key recognition sites of molecules such as RIG-I, MDA-5, and Dicer to silence viral dsRNA in infection.

Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression.,Kimberlin CR, Bornholdt ZA, Li S, Woods VL Jr, Macrae IJ, Saphire EO Proc Natl Acad Sci U S A. 2009 Dec 14. PMID:20018665^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kimberlin CR, Bornholdt ZA, Li S, Woods VL Jr, Macrae IJ, Saphire EO. Ebolavirus VP35 uses a bimodal strategy to bind dsRNA for innate immune suppression. Proc Natl Acad Sci U S A. 2009 Dec 14. PMID:20018665

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ks8"

@@ Line 1: / Line 1: @@
 ==Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA==
 <StructureSection load='3ks8' size='340' side='right' caption='[[3ks8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ks4|3ks4]], [[3fke|3fke]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">REBOVgp2, VP35 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=129003 EBORE])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ks8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ks8 OCA], [http://pdbe.org/3ks8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ks8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ks8 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ks8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ks8 OCA], [http://pdbe.org/3ks8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ks8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ks8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ks8 ProSAT]</span></td></tr>
 </table>
 == Function ==

3ks8

From Proteopedia

Revision as of 08:40, 1 November 2017

Crystal structure of Reston ebolavirus VP35 RNA binding domain in complex with 18bp dsRNA

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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