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1nyh

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Revision as of 09:45, 1 November 2017

Crystal Structure of the Coiled-coil Dimerization Motif of Sir4

Structural highlights

1nyh is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	SIR4 (ATCC 18824)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SIR4_YEAST] The proteins SIR1 through SIR4 are required for transcriptional repression of the silent mating type loci, HML and HMR. The proteins SIR2 through SIR4 repress mulitple loci by modulating chromatin structure. Involves the compaction of chromatin fiber into a more condensed form.^[1]

Publication Abstract from PubMed

The yeast silent information regulators Sir2, Sir3, and Sir4 physically interact with one another to establish a transcriptionally silent state by forming repressive chromatin structures. The Sir4 protein contains binding sites for both Sir2 and Sir3, and these protein-protein interactions are required for gene silencing. Here, we report the X-ray structure of the coiled-coil dimerization motif within the C-terminus of Sir4 and show that it forms a stable 1:1 complex with a dimeric fragment of Sir3 (residues 464-978). We have identified a cluster of residues on the surface of the Sir4 coiled coil required for specific interactions with Sir3. The histone deacetylase Sir2 can also bind to this complex, forming a ternary complex with the truncated Sir3 and Sir4 proteins. The dual interactions of Sir4 with Sir3 and Sir2 suggest a physical basis for recruiting Sir3 to chromatin by virtue of its interactions with Sir4 and with deacetylated histones in chromatin.

Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3.,Chang JF, Hall BE, Tanny JC, Moazed D, Filman D, Ellenberger T Structure. 2003 Jun;11(6):637-49. PMID:12791253^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bupp JM, Martin AE, Stensrud ES, Jaspersen SL. Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3. J Cell Biol. 2007 Dec 3;179(5):845-54. Epub 2007 Nov 26. PMID:18039933 doi:http://dx.doi.org/10.1083/jcb.200706040
↑ Chang JF, Hall BE, Tanny JC, Moazed D, Filman D, Ellenberger T. Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3. Structure. 2003 Jun;11(6):637-49. PMID:12791253

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nyh"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Coiled-coil Dimerization Motif of Sir4==
 <StructureSection load='1nyh' size='340' side='right' caption='[[1nyh]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1nyh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NYH FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SIR4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyh OCA], [http://pdbe.org/1nyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nyh RCSB], [http://www.ebi.ac.uk/pdbsum/1nyh PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyh OCA], [http://pdbe.org/1nyh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nyh RCSB], [http://www.ebi.ac.uk/pdbsum/1nyh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nyh ProSAT]</span></td></tr>
 </table>
 == Function ==

1nyh

From Proteopedia

Revision as of 09:45, 1 November 2017

Crystal Structure of the Coiled-coil Dimerization Motif of Sir4

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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