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5lv3

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'''Unreleased structure'''
 
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The entry 5lv3 is ON HOLD until Paper Publication
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==Crystal structure of mouse CARM1 in complex with ligand LH1561Br==
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<StructureSection load='5lv3' size='340' side='right' caption='[[5lv3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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Authors: Cura, V., Marechal, N., Troffer-Charlier, N., Wurtz, J.M., Bonnefond, L., Cavarelli, J.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5lv3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LV3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5LV3 FirstGlance]. <br>
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Description: Crystal structure of mouse CARM1 in complex with ligand LH1561Br
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LHF:5-[[2-[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]ethylamino]methyl]-4-azanyl-1-[2-(4-bromanylphenoxy)ethyl]pyrimidin-2-one'>LHF</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
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[[Category: Unreleased Structures]]
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Carm1, Prmt4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_I_protein_arginine_methyltransferase Type I protein arginine methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.319 2.1.1.319] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5lv3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lv3 OCA], [http://pdbe.org/5lv3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5lv3 RCSB], [http://www.ebi.ac.uk/pdbsum/5lv3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5lv3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/CARM1_MOUSE CARM1_MOUSE]] Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.<ref>PMID:10381882</ref> <ref>PMID:11341840</ref> <ref>PMID:11701890</ref> <ref>PMID:11713257</ref> <ref>PMID:11983685</ref> <ref>PMID:11997499</ref> <ref>PMID:12756295</ref> <ref>PMID:14966289</ref> <ref>PMID:15186775</ref> <ref>PMID:15616592</ref> <ref>PMID:16322096</ref> <ref>PMID:17218272</ref> <ref>PMID:17882261</ref> <ref>PMID:18188184</ref> <ref>PMID:19843527</ref> <ref>PMID:19897492</ref> <ref>PMID:21138967</ref>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Lk3 transgenic mice]]
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[[Category: Type I protein arginine methyltransferase]]
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[[Category: Arimondo, P]]
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[[Category: Bonnefond, L]]
[[Category: Cavarelli, J]]
[[Category: Cavarelli, J]]
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[[Category: Cura, V]]
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[[Category: Halby, L]]
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[[Category: Marechal, N]]
[[Category: Troffer-Charlier, N]]
[[Category: Troffer-Charlier, N]]
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[[Category: Bonnefond, L]]
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[[Category: Catalytic domain]]
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[[Category: Marechal, N]]
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[[Category: Chromatin regulator]]
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[[Category: Wurtz, J.M]]
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[[Category: Mrna processing]]
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[[Category: Cura, V]]
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[[Category: Mrna splicing]]
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[[Category: Nucleus]]
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[[Category: Protein arginine methyltransferase]]
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[[Category: S-adenosyl-l-methionine]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transferase]]

Revision as of 14:18, 15 November 2017

Crystal structure of mouse CARM1 in complex with ligand LH1561Br

5lv3, resolution 1.80Å

Drag the structure with the mouse to rotate

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