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1qmj

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Revision as of 07:06, 16 November 2017

CG-16, a homodimeric agglutinin from chicken liver

Structural highlights

1qmj is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[LEG6_CHICK] This protein binds beta-galactoside. Its physiological function is not yet known. It may be involved in the regulation of differentiation.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Differential developmental regulation of expression, fine-specificity differences in ligand recognition and disparate capacity for homodimerization are characteristics of the two currently known proto-type chicken galectins. The X-ray crystal structure of the first avian galectin, the homodimeric agglutinin from chicken liver (CG-16), has been solved in the absence of ligand in two crystal forms. Although the arrangement of lectin dimers in the two crystals is different, the structure of the monomers and their association into the extended beta-sandwich that characterises the dimer are virtually identical. The fold establishes a beta-sandwich motif composed of a five-stranded and a six-stranded beta-sheet evocative of proto-type mammalian galectins. The carbohydrate-binding site is occupied by six water molecules that take the place of the sugar in the complex. They help to stabilise in the absence of the ligand the spatial arrangement of the amino acid side-chains involved in sugar recognition. Docking of N-acetyllactosamine into the binding site reveals that three of these water molecules, which are in direct contact with the protein, occupy positions equivalent to the key sugar hydroxyl groups, namely the hydroxyls at positions 4 and 6 of the galactose unit and at position 3 of the N-acetylglucosamine unit. Crystallographic data are fully consistent with the binding features in solution previously derived from chemical mapping with deoxy, fluoro and O-methyl derivatives and laser photo-CIDNP (chemically induced dynamic nuclear polarisation) studies. The possible molecular basis for the monomeric character of the chicken intestinal galectin as well as potential mechanisms of oxidative inactivation by disulphide bridging are evaluated on the basis of the given structural information concerning the CG-16 dimer interface and the cysteine residues, respectively.

The 2.15 A crystal structure of CG-16, the developmentally regulated homodimeric chicken galectin.,Varela PF, Solis D, Diaz-Maurino T, Kaltner H, Gabius HJ, Romero A J Mol Biol. 1999 Nov 26;294(2):537-49. PMID:10610778^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Varela PF, Solis D, Diaz-Maurino T, Kaltner H, Gabius HJ, Romero A. The 2.15 A crystal structure of CG-16, the developmentally regulated homodimeric chicken galectin. J Mol Biol. 1999 Nov 26;294(2):537-49. PMID:10610778 doi:10.1006/jmbi.1999.3273

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qmj"

@@ Line 1: / Line 1: @@
 ==CG-16, a homodimeric agglutinin from chicken liver==
 <StructureSection load='1qmj' size='340' side='right' caption='[[1qmj]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1qmj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QMJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1qmj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QMJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QMJ FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmj OCA], [http://pdbe.org/1qmj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qmj RCSB], [http://www.ebi.ac.uk/pdbsum/1qmj PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qmj OCA], [http://pdbe.org/1qmj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qmj RCSB], [http://www.ebi.ac.uk/pdbsum/1qmj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qmj ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Chick]]
+[[Category: Gallus gallus]]
 [[Category: Diaz-Maurino, T]]
 [[Category: Gabius, H J]]

1qmj

From Proteopedia

Revision as of 07:06, 16 November 2017

CG-16, a homodimeric agglutinin from chicken liver

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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