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5frx

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Revision as of 13:20, 16 November 2017

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-nitrophenol

Structural highlights

5frx is a 2 chain structure with sequence from alcaligenes_eutrophus Atcc 17697 alcaligenes eutrophus . Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	5fru, 5frv, 5frw, 5fry, 5frz, 5fs0
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Positive phenol-degradative gene regulator (PoxR) is a sigma(54)-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering.

Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR.,Patil VV, Park KH, Lee SG, Woo E Structure. 2016 Apr 5;24(4):624-30. doi: 10.1016/j.str.2016.03.006. PMID:27050690^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Patil VV, Park KH, Lee SG, Woo E. Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR. Structure. 2016 Apr 5;24(4):624-30. doi: 10.1016/j.str.2016.03.006. PMID:27050690 doi:http://dx.doi.org/10.1016/j.str.2016.03.006

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5frx"

@@ Line 3: / Line 3: @@
 <StructureSection load='5frx' size='340' side='right' caption='[[5frx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5frx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FRX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5frx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_17697_[[alcaligenes_eutrophus]] Atcc 17697 [[alcaligenes eutrophus]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FRX FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NPO:P-NITROPHENOL'>NPO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fru|5fru]], [[5frv|5frv]], [[5frw|5frw]], [[5fry|5fry]], [[5frz|5frz]], [[5fs0|5fs0]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5frx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frx OCA], [http://pdbe.org/5frx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5frx RCSB], [http://www.ebi.ac.uk/pdbsum/5frx PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5frx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frx OCA], [http://pdbe.org/5frx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5frx RCSB], [http://www.ebi.ac.uk/pdbsum/5frx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5frx ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">

5frx

From Proteopedia

Revision as of 13:20, 16 November 2017

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-nitrophenol

Structural highlights

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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