2bdz
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1yal|1YAL]], [[1gece|1GECE]], [[1ppo|1PPO]], [[1meg|1MEG]], [[1pcia|1PCIA]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bdz OCA], [http://www.ebi.ac.uk/pdbsum/2bdz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bdz RCSB]</span> | ||
}} | }} | ||
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[[Category: Oliver-Salvador, M C.]] | [[Category: Oliver-Salvador, M C.]] | ||
[[Category: Soriano-Garcia, M.]] | [[Category: Soriano-Garcia, M.]] | ||
| - | [[Category: E64]] | ||
[[Category: cysteine protease]] | [[Category: cysteine protease]] | ||
[[Category: mexicain]] | [[Category: mexicain]] | ||
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[[Category: peptidase_c1]] | [[Category: peptidase_c1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:05:00 2008'' |
Revision as of 23:05, 30 March 2008
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| , resolution 2.100Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Related: | 1YAL, 1GECE, 1PPO, 1MEG, 1PCIA
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Mexicain from Jacaratia mexicana
Overview
Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.
About this Structure
2BDZ is a Single protein structure of sequence from Jacaratia mexicana. Full crystallographic information is available from OCA.
Reference
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326
Page seeded by OCA on Mon Mar 31 02:05:00 2008
