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1bk8

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Revision as of 11:13, 22 November 2017

DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES

Structural highlights

1bk8 is a 1 chain structure with sequence from Aesculus hippocastanum. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DEF1_AESHI] Possesses antimicrobial activity sensitive to inorganic cations. Binds specifically to the fungal plasma membrane. Has no inhibitory effect on insect gut alpha-amylase.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Aesculus hippocastanum antimicrobial protein 1 (Ah-AMP1) is a plant defensin isolated from horse chestnuts. The plant defensins have been divided in several subfamilies according to their amino acid sequence homology. Ah-AMP1, belonging to subfamily A2, inhibits growth of a broad range of fungi. So far, a three-dimensional structure has been determined only for members of subfamilies A3 and B2. In order to understand activity and specificity of these plant defensins, the structure of a protein belonging to subfamily A2 is needed. We report the three-dimensional solution structure of Ah-AMP1 as determined from two-dimensional 1H nuclear magnetic resonance data. The structure features all the characteristics of the "cysteine-stabilized alpha beta-motif." A comparison of the structure, the electrostatic potential surface and regions important for interaction with the fungal receptor, is made with Rs-AFP1 (plant defensin of subfamily A3). Thus, residues important for activity and specificity have been assigned.

The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance.,Fant F, Vranken WF, Borremans FA Proteins. 1999 Nov 15;37(3):388-403. PMID:10591099^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Thevissen K, Osborn RW, Acland DP, Broekaert WF. Specific binding sites for an antifungal plant defensin from Dahlia (Dahlia merckii) on fungal cells are required for antifungal activity. Mol Plant Microbe Interact. 2000 Jan;13(1):54-61. PMID:10656585 doi:http://dx.doi.org/10.1094/MPMI.2000.13.1.54
↑ Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S, Van Leuven F, Attenborough S, Rees SB, Broekaert WF. Isolation and characterisation of plant defensins from seeds of Asteraceae, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett. 1995 Jul 17;368(2):257-62. PMID:7628617
↑ Fant F, Vranken WF, Borremans FA. The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance. Proteins. 1999 Nov 15;37(3):388-403. PMID:10591099

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bk8"

@@ Line 1: / Line 1: @@
 ==DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES==
 <StructureSection load='1bk8' size='340' side='right' caption='[[1bk8]], [[NMR_Ensembles_of_Models | 25 NMR models]]' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1bk8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aesculus_hippocastanum Aesculus hippocastanum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1BK8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bk8 OCA], [http://pdbe.org/1bk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1bk8 PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bk8 OCA], [http://pdbe.org/1bk8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1bk8 RCSB], [http://www.ebi.ac.uk/pdbsum/1bk8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1bk8 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 26: / Line 27: @@
 </div>
 <div class="pdbe-citations 1bk8" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Plant defensin|Plant defensin]]
 == References ==
 <references/>

1bk8

From Proteopedia

Revision as of 11:13, 22 November 2017

DETERMINATION OF THE THREE-DIMENSIONAL SOLUTION STRUCTURE OF AESCULUS HIPPOCASTANUM ANTIMICROBIAL PROTEIN 1 (AH-AMP1) BY 1H NMR, 25 STRUCTURES

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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