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5nxt
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Wobble base paired RNA double helix== | |
| + | <StructureSection load='5nxt' size='340' side='right' caption='[[5nxt]], [[Resolution|resolution]] 1.38Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5nxt]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NXT FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nxt OCA], [http://pdbe.org/5nxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nxt RCSB], [http://www.ebi.ac.uk/pdbsum/5nxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nxt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Wobble base pairs are critical in various physiological functions and have been linked to local structural perturbations in double helical structures of nucleic acids. We report a 1.38-A resolution crystal structure of an antiparallel octadecamer RNA double helix in overall A conformation, which includes a unique, central stretch of six consecutive wobble base pairs (W helix) with two G.U and four rare C.A(+) wobble pairs. Four adenines within the W helix are N1-protonated and wobble-base-paired with the opposing cytosine through two regular hydrogen bonds. Combined with the two G.U pairs, the C.A(+) base pairs facilitate formation of a half turn of W-helical RNA flanked by six regular Watson-Crick base pairs in standard A conformation on either side. RNA melting experiments monitored by differential scanning calorimetry, UV and circular dichroism spectroscopy demonstrate that the RNA octadecamer undergoes a pH-induced structural transition which is consistent with the presence of a duplex with C.A(+) base pairs at acidic pH. Our crystal structure provides a first glimpse of an RNA double helix based entirely on wobble base pairs with possible applications in RNA or DNA nanotechnology and pH biosensors. | ||
| - | + | A Novel Form of RNA Double Helix Based on G.U and C.A(+) Wobble Base Pairing.,Garg A, Heinemann U RNA. 2017 Nov 9. pii: rna.064048.117. doi: 10.1261/rna.064048.117. PMID:29122970<ref>PMID:29122970</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5nxt" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Garg, A]] | ||
| + | [[Category: Heinemann, U]] | ||
| + | [[Category: Double helix]] | ||
| + | [[Category: N1-adenine protonation]] | ||
| + | [[Category: Rna]] | ||
| + | [[Category: Wobble pair]] | ||
Revision as of 06:08, 29 November 2017
Wobble base paired RNA double helix
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