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5v8e
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5v8e is ON HOLD Authors: Sychantha, D., Little, D.J., Chapman, R.N., Boons, G.J., Robinson, H., Howell, P.L., Clarke, A.J. Description: Structure o...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Bacillus cereus PatB1== | |
| + | <StructureSection load='5v8e' size='340' side='right' caption='[[5v8e]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5v8e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacc1 Bacc1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5V8E FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5v8d|5v8d]]</td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BCE_0974 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=222523 BACC1])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8e OCA], [http://pdbe.org/5v8e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5v8e RCSB], [http://www.ebi.ac.uk/pdbsum/5v8e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1. | ||
| - | + | PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.,Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ Nat Chem Biol. 2017 Oct 30. pii: nchembio.2509. doi: 10.1038/nchembio.2509. PMID:29083419<ref>PMID:29083419</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5v8e" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Clarke, A | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacc1]] | ||
| + | [[Category: Boons, G J]] | ||
| + | [[Category: Chapman, R N]] | ||
| + | [[Category: Clarke, A J]] | ||
| + | [[Category: Howell, P L]] | ||
| + | [[Category: Little, D J]] | ||
[[Category: Robinson, H]] | [[Category: Robinson, H]] | ||
[[Category: Sychantha, D]] | [[Category: Sychantha, D]] | ||
| - | [[Category: | + | [[Category: Acetyltransferase]] |
| - | [[Category: | + | [[Category: Cell wall]] |
| + | [[Category: Sgnh hydrolase-like]] | ||
| + | [[Category: Transferase]] | ||
| + | [[Category: Unknown function]] | ||
Current revision
Structure of Bacillus cereus PatB1
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