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6ezq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6ezq is ON HOLD Authors: Wenskowsky, L., Liesum, A., Schreuder, H.A. Description: human Serum Albumin complexed with NBD-C12 fatty acid [[Category:...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==human Serum Albumin complexed with NBD-C12 fatty acid== | |
| + | <StructureSection load='6ezq' size='340' side='right' caption='[[6ezq]], [[Resolution|resolution]] 2.39Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ezq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EZQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EZQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C7K:12-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]dodecanoic+acid'>C7K</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ezq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ezq OCA], [http://pdbe.org/6ezq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ezq RCSB], [http://www.ebi.ac.uk/pdbsum/6ezq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ezq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:[http://omim.org/entry/103600 103600]]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.<ref>PMID:8048949</ref> <ref>PMID:7852505</ref> <ref>PMID:9329347</ref> <ref>PMID:9589637</ref> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ALBU_HUMAN ALBU_HUMAN]] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.<ref>PMID:19021548</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A single high-affinity fatty acid binding site in the important human transport protein serum albumin (HSA) is identified and characterized using a NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl)-C12 fatty acid. This ligand exhibits a 1:1 binding stoichiometry in its HSA complex with high site-specificity. The complex dissociation constant is determined by titration experiments as well as radioactive equilibrium dialysis. Competition experiments with known HSA-binding drugs warfarin and ibuprofen confirm the new binding site to be different from Sudlow-sites I and II. These binding studies are extended to other albumin binders and fatty acid derivatives. Furthermore an X-ray crystal structure allows locating the binding site in HSA subdomain IIA. The knowledge about this novel HSA site will be important for drug depot development and for understanding drug-protein, which are important prerequisites for modulation of drug pharmacokinetics. | ||
| - | + | Identification and Characterization of a Single High-Affinity Fatty Acid Binding Site in Human Serum Albumin.,Petry S, Wenskowsky L, Schreuder H, Derdau V, Matter H, Volkmar J, Opatz T Angew Chem Int Ed Engl. 2017 Nov 28. doi: 10.1002/anie.201710437. PMID:29193545<ref>PMID:29193545</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6ezq" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Liesum, A]] | [[Category: Liesum, A]] | ||
| + | [[Category: Schreuder, H A]] | ||
[[Category: Wenskowsky, L]] | [[Category: Wenskowsky, L]] | ||
| - | [[Category: | + | [[Category: Fatty acid binding site human serum albumin nbd label drug interaction]] |
| + | [[Category: Transport protein]] | ||
Current revision
human Serum Albumin complexed with NBD-C12 fatty acid
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