2cj8
From Proteopedia
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|PDB= 2cj8 |SIZE=350|CAPTION= <scene name='initialview01'>2cj8</scene>, resolution 2.38Å | |PDB= 2cj8 |SIZE=350|CAPTION= <scene name='initialview01'>2cj8</scene>, resolution 2.38Å | ||
|SITE= <scene name='pdbsite=AC1:Iod+Binding+Site+For+Chain+B'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Iod+Binding+Site+For+Chain+B'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=IOD:IODIDE ION'>IOD</scene> | + | |LIGAND= <scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cj8 OCA], [http://www.ebi.ac.uk/pdbsum/2cj8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cj8 RCSB]</span> | ||
}} | }} | ||
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[[Category: Hothorn, M.]] | [[Category: Hothorn, M.]] | ||
[[Category: Scheffzek, K.]] | [[Category: Scheffzek, K.]] | ||
| - | [[Category: IOD]] | ||
[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
[[Category: helical hairpin]] | [[Category: helical hairpin]] | ||
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[[Category: protein binding]] | [[Category: protein binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:22:15 2008'' |
Revision as of 23:22, 30 March 2008
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| , resolution 2.38Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A CELL WALL INVERTASE INHIBITOR FROM TOBACCO (PH 9.5)
Overview
Plant acid invertases catalyse the breakdown of sucrose. Their activity is tightly regulated through interaction with specific protein inhibitors. The complex between the cell-wall invertase inhibitor Nt-CIF and its target enzyme is stable only at acidic pH, as found in the plant cell wall. Since the pH in this compartment can be modulated between pH 4 and 6 in planta, the rapid dissociation of the inhibitor-enzyme complex at neutral pH may represent a regulatory event. Here, it is analyzed whether the inhibitory component undergoes structural rearrangements upon changes in the pH environment. Six crystal forms grown at pH 4.6-9.5 and diffracting up to 1.63 Angstrom indicate only small structural changes in CIF. This suggests that complex dissociation at neutral pH is mediated either by rearrangements in the enzyme or by a complex pattern of surface charges in the inhibitor-enzyme binding interface.
About this Structure
2CJ8 is a Single protein structure of sequence from Nicotiana tabacum. Full crystallographic information is available from OCA.
Reference
Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range., Hothorn M, Scheffzek K, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):665-70. Epub 2006, May 12. PMID:16699194
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