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1fn9

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Revision as of 09:23, 27 December 2017

CRYSTAL STRUCTURE OF THE REOVIRUS OUTER CAPSID PROTEIN SIGMA 3

Structural highlights

1fn9 is a 2 chain structure with sequence from Reovirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystallographically determined structure of the reovirus outer capsid protein sigma3 reveals a two-lobed structure organized around a long central helix. The smaller of the two lobes includes a CCHC zinc-binding site. Residues that vary between strains and serotypes lie mainly on one surface of the protein; residues on the opposite surface are conserved. From a fit of this model to a reconstruction of the whole virion from electron cryomicroscopy, we propose that each sigma3 subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards. The surface containing variable residues faces solvent. The crystallographic asymmetric unit contains two sigma3 subunits, tightly associated as a dimer. One broad surface of the dimer has a positively charged surface patch, which extends across the dyad. In infected cells, sigma3 binds dsRNA and inhibits the interferon response. The location and extent of the positively charged surface patch suggest that the dimer is the RNA-binding form of sigma3.

Structure of the reovirus outer capsid and dsRNA-binding protein sigma3 at 1.8 A resolution.,Olland AM, Jane-Valbuena J, Schiff LA, Nibert ML, Harrison SC EMBO J. 2001 Mar 1;20(5):979-89. PMID:11230122^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Olland AM, Jane-Valbuena J, Schiff LA, Nibert ML, Harrison SC. Structure of the reovirus outer capsid and dsRNA-binding protein sigma3 at 1.8 A resolution. EMBO J. 2001 Mar 1;20(5):979-89. PMID:11230122 doi:10.1093/emboj/20.5.979

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fn9"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF THE REOVIRUS OUTER CAPSID PROTEIN SIGMA 3==
 <StructureSection load='1fn9' size='340' side='right' caption='[[1fn9]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1fn9]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Reovirus Reovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FN9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1FN9 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn9 OCA], [http://pdbe.org/1fn9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fn9 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn9 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fn9 OCA], [http://pdbe.org/1fn9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1fn9 RCSB], [http://www.ebi.ac.uk/pdbsum/1fn9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1fn9 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 10: / Line 11: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn9_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fn/1fn9_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 25: / Line 26: @@
 </div>
 <div class="pdbe-citations 1fn9" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Virus coat protein|Virus coat protein]]
 == References ==
 <references/>

1fn9

From Proteopedia

Revision as of 09:23, 27 December 2017

CRYSTAL STRUCTURE OF THE REOVIRUS OUTER CAPSID PROTEIN SIGMA 3

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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