2cw0
From Proteopedia
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|PDB= 2cw0 |SIZE=350|CAPTION= <scene name='initialview01'>2cw0</scene>, resolution 3.3Å | |PDB= 2cw0 |SIZE=350|CAPTION= <scene name='initialview01'>2cw0</scene>, resolution 3.3Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2cw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cw0 OCA], [http://www.ebi.ac.uk/pdbsum/2cw0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2cw0 RCSB]</span> | ||
}} | }} | ||
| Line 40: | Line 43: | ||
[[Category: Tuske, S.]] | [[Category: Tuske, S.]] | ||
[[Category: Wang, X.]] | [[Category: Wang, X.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: ZN]] | ||
[[Category: bent-bridge helix]] | [[Category: bent-bridge helix]] | ||
[[Category: rna polymerase holoenzyme]] | [[Category: rna polymerase holoenzyme]] | ||
[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:27:05 2008'' |
Revision as of 23:27, 30 March 2008
| |||||||
| , resolution 3.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Thermus thermophilus RNA polymerase holoenzyme at 3.3 angstroms resolution
Overview
We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
About this Structure
2CW0 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation., Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E, Cell. 2005 Aug 26;122(4):541-52. PMID:16122422
Page seeded by OCA on Mon Mar 31 02:27:05 2008
Categories: DNA-directed RNA polymerase | Protein complex | Thermus thermophilus | Arnold, E. | Birktoft, J J. | Borukhov, S. | Dharia, C. | Ebright, R H. | Hudson, B. | Ismail, S. | Jr., A D.Clark. | Laptenko, O. | Lee, J. | Leroy, O. | Mukhopadhyay, J. | Napoli, A. | Sarafianos, S G. | Sineva, E. | Tuske, S. | Wang, X. | Bent-bridge helix | Rna polymerase holoenzyme | Transcription
