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2czt
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Prostaglandin-D_synthase Prostaglandin-D synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.99.2 5.3.99.2] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2czu|2CZU]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2czt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2czt OCA], [http://www.ebi.ac.uk/pdbsum/2czt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2czt RCSB]</span> | ||
}} | }} | ||
| Line 39: | Line 42: | ||
[[Category: structural genomic]] | [[Category: structural genomic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:28:25 2008'' |
Revision as of 23:28, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Prostaglandin-D synthase, with EC number 5.3.99.2 | ||||||
| Related: | 2CZU
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
lipocalin-type prostaglandin D synthase
Overview
Lipocalin-type prostaglandin D synthase is the key enzyme for the production of prostaglandin D(2), a potent endogenous somnogen, in the brain. We cloned, produced, and crystallized the native enzyme and selenomethionyl Cys(65)Ala mutants of the recombinant mouse protein by the hanging drop vapor-diffusion method with both malonate and citrate as precipitants. The native crystals obtained with malonate belong to orthorhombic space group P2(1)2(1)2(1) with lattice constants a = 46.2, b = 66.8, and c = 105.3 A. The selenomethionyl crystals obtained with citrate belong to orthorhombic space group C222(1) with lattice constants a = 45.5, b = 66.8, and c = 104.5 A. The native crystals diffracted beyond 2.1 A resolution.
About this Structure
2CZT is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Cloning, expression, crystallization, and preliminary X-ray analysis of recombinant mouse lipocalin-type prostaglandin D synthase, a somnogen-producing enzyme., Irikura D, Kumasaka T, Yamamoto M, Ago H, Miyano M, Kubata KB, Sakai H, Hayaishi O, Urade Y, J Biochem. 2003 Jan;133(1):29-32. PMID:12761195
Page seeded by OCA on Mon Mar 31 02:28:25 2008
Categories: Mus musculus | Prostaglandin-D synthase | Single protein | Ago, H. | Aritake, K. | Hayaishi, O. | Inoue, T. | Irikura, D. | Kumasaka, T. | Miyano, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Urade, Y. | Yamamoto, M. | C2221 native | Lipocalin | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
