1hqc

From Proteopedia

(Difference between revisions)

Revision as of 09:17, 10 January 2018

STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8

Structural highlights

1hqc is a 2 chain structure with sequence from "flavobacterium_thermophilum"_yoshida_and_oshima_1971 "flavobacterium thermophilum" yoshida and oshima 1971. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	RUVB ("Flavobacterium thermophilum" Yoshida and Oshima 1971)
Activity:	Adenosinetriphosphatase, with EC number 3.6.1.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RUVB_THET8] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg(2+)-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hydrolysis. DNA is likely to interact with a large basic cleft, which encompasses the ATP-binding pocket and domain boundaries, whereas the junction-recognition protein RuvA may bind a flexible beta-hairpin protruding from the N-terminal domain. The structures of two subunits, related by a noncrystallographic pseudo-2-fold axis, imply that conformational changes of motor protein coupled with ATP hydrolysis may reflect motility essential for its translocation around double-stranded DNA.

Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8.,Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:11171970^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yamada K, Fukuoh A, Iwasaki H, Shinagawa H. Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions. Mol Gen Genet. 1999 Jul;261(6):1001-11. PMID:10485292
↑ Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K. Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:11171970 doi:10.1073/pnas.031470598

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hqc"

@@ Line 1: / Line 1: @@
 ==STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8==
 <StructureSection load='1hqc' size='340' side='right' caption='[[1hqc]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RUVB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 "Flavobacterium thermophilum" Yoshida and Oshima 1971])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqc OCA], [http://pdbe.org/1hqc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hqc RCSB], [http://www.ebi.ac.uk/pdbsum/1hqc PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hqc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hqc OCA], [http://pdbe.org/1hqc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1hqc RCSB], [http://www.ebi.ac.uk/pdbsum/1hqc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1hqc ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hqc_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hq/1hqc_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 30: @@
 </div>
 <div class="pdbe-citations 1hqc" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Helicase|Helicase]]
 == References ==
 <references/>

1hqc

From Proteopedia

Revision as of 09:17, 10 January 2018

STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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