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1jqt

From Proteopedia

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Revision as of 08:54, 17 January 2018

Fitting of L11 protein in the low resolution cryo-EM map of E.coli 70S ribosome

Structural highlights

1jqt is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1eg0
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RL11_THEMA] This protein binds directly to 23S ribosomal RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

L11 protein is located at the base of the L7/L12 stalk of the 50 S subunit of the Escherichia coli ribosome. Because of the flexible nature of the region, recent X-ray crystallographic studies of the 50 S subunit failed to locate the N-terminal domain of the protein. We have determined the position of the complete L11 protein by comparing a three-dimensional cryo-EM reconstruction of the 70 S ribosome, isolated from a mutant lacking ribosomal protein L11, with the three-dimensional map of the wild-type ribosome. Fitting of the X-ray coordinates of L11-23 S RNA complex and EF-G into the cryo-EM maps combined with molecular modeling, reveals that, following EF-G-dependent GTP hydrolysis, domain V of EF-G intrudes into the cleft between the 23 S ribosomal RNA and the N-terminal domain of L11 (where the antibiotic thiostrepton binds), causing the N-terminal domain to move and thereby inducing the formation of the arc-like connection with the G' domain of EF-G. The results provide a new insight into the mechanism of EF-G-dependent translocation.

Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation.,Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Agrawal RK, Linde J, Sengupta J, Nierhaus KH, Frank J. Localization of L11 protein on the ribosome and elucidation of its involvement in EF-G-dependent translocation. J Mol Biol. 2001 Aug 24;311(4):777-87. PMID:11518530 doi:http://dx.doi.org/10.1006/jmbi.2001.4907

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1jqt"

@@ Line 1: / Line 1: @@
 ==Fitting of L11 protein in the low resolution cryo-EM map of E.coli 70S ribosome==
-<StructureSection load='1jqt' size='340' side='right' caption='[[1jqt]]' scene=''>
+<StructureSection load='1jqt' size='340' side='right' caption='[[1jqt]], [[Resolution|resolution]] 18.00&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[1jqt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JQT FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eg0|1eg0]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqt OCA], [http://pdbe.org/1jqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jqt RCSB], [http://www.ebi.ac.uk/pdbsum/1jqt PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jqt OCA], [http://pdbe.org/1jqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jqt RCSB], [http://www.ebi.ac.uk/pdbsum/1jqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jqt ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqt_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jqt_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 27: / Line 28: @@
 </div>
 <div class="pdbe-citations 1jqt" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Ribosomal protein L11|Ribosomal protein L11]]
 == References ==
 <references/>

1jqt

From Proteopedia

Revision as of 08:54, 17 January 2018

Fitting of L11 protein in the low resolution cryo-EM map of E.coli 70S ribosome

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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