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2dgk
From Proteopedia
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|PDB= 2dgk |SIZE=350|CAPTION= <scene name='initialview01'>2dgk</scene>, resolution 1.90Å | |PDB= 2dgk |SIZE=350|CAPTION= <scene name='initialview01'>2dgk</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5'-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Glutamate_decarboxylase Glutamate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.15 4.1.1.15] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_decarboxylase Glutamate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.15 4.1.1.15] </span> |
|GENE= gadB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= gadB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1pmm|1PMM]], [[1pmo|1PMO]], [[2dgl|2DGL]], [[2dgm|2DGM]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dgk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dgk OCA], [http://www.ebi.ac.uk/pdbsum/2dgk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2dgk RCSB]</span> | ||
}} | }} | ||
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[[Category: Gruetter, M G.]] | [[Category: Gruetter, M G.]] | ||
[[Category: Gut, H.]] | [[Category: Gut, H.]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: PLP]] | ||
| - | [[Category: SO4]] | ||
[[Category: autoinhibition]] | [[Category: autoinhibition]] | ||
[[Category: gadb]] | [[Category: gadb]] | ||
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[[Category: substituted aldamine]] | [[Category: substituted aldamine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:34:22 2008'' |
Revision as of 23:34, 30 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | gadB (Escherichia coli) | ||||||
| Activity: | Glutamate decarboxylase, with EC number 4.1.1.15 | ||||||
| Related: | 1PMM, 1PMO, 2DGL, 2DGM
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an N-terminal deletion mutant of Escherichia coli GadB in an autoinhibited state (aldamine)
Overview
Escherichia coli and other enterobacteria exploit the H+ -consuming reaction catalysed by glutamate decarboxylase to survive the stomach acidity before reaching the intestine. Here we show that chloride, extremely abundant in gastric secretions, is an allosteric activator producing a 10-fold increase in the decarboxylase activity at pH 5.6. Cooperativity and sensitivity to chloride were lost when the N-terminal 14 residues, involved in the formation of two triple-helix bundles, were deleted by mutagenesis. X-ray structures, obtained in the presence of the substrate analogue acetate, identified halide-binding sites at the base of each N-terminal helix, showed how halide binding is responsible for bundle stability and demonstrated that the interconversion between active and inactive forms of the enzyme is a stepwise process. We also discovered an entirely novel structure of the cofactor pyridoxal 5'-phosphate (aldamine) to be responsible for the reversibly inactivated enzyme. Our results link the entry of chloride ions, via the H+/Cl- exchange activities of ClC-ec1, to the trigger of the acid stress response in the cell when the intracellular proton concentration has not yet reached fatal values.
About this Structure
2DGK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Escherichia coli acid resistance: pH-sensing, activation by chloride and autoinhibition in GadB., Gut H, Pennacchietti E, John RA, Bossa F, Capitani G, De Biase D, Grutter MG, EMBO J. 2006 Jun 7;25(11):2643-51. Epub 2006 May 4. PMID:16675957
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