DNA repair protein

From Proteopedia

Revision as of 09:03, 15 February 2018

spinqualitylabelsall models Structure of yeast Rad4 Grey) complex with Rad23 (green) and DNA (PDB entry 2qsg) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function DNA repair protein Rad4 is a yeast protein involved in DNA damage recognition in nucleotide excision repair[1]. Rad4 is an ortholog of the XPC protein. Rad4 opens up damaged DNA by inserting β-hairpin into the duplex and flipping out two damage-containing nucleotide pairs[2]. Disease Mutations in the nucleotide excision repair pathway can cause the xeroderma pigmentosum skin cancer predisposition syndrome[3]. Structural highlights

3D Structures of DNA repair protein Rad4

Updated on 15-February-2018

2qsf – yRad4 + Rad23 - yeast
2qsg, 2qsh, 4yir – yRad4 + Rad23 + DNA
2m14 – yRad4 internal UBA domain + RNA polymerase II TFB1 - NMR
4bu0, 4bu1 – fyRad4 BRCT1 domain + phosphopeptide – fission yeast

References

1. ↑ den Dulk B, Brandsma JA, Brouwer J. The Rad4 homologue YDR314C is essential for strand-specific repair of RNA polymerase I-transcribed rDNA in Saccharomyces cerevisiae. Mol Microbiol. 2005 Jun;56(6):1518-26. doi: 10.1111/j.1365-2958.2005.04607.x. PMID:15916602 doi:http://dx.doi.org/10.1111/j.1365-2958.2005.04607.x
2. ↑ Velmurugu Y, Chen X, Slogoff Sevilla P, Min JH, Ansari A. Twist-open mechanism of DNA damage recognition by the Rad4/XPC nucleotide excision repair complex. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):E2296-305. doi:, 10.1073/pnas.1514666113. Epub 2016 Mar 31. PMID:27035942 doi:http://dx.doi.org/10.1073/pnas.1514666113
3. ↑ Min JH, Pavletich NP. Recognition of DNA damage by the Rad4 nucleotide excision repair protein. Nature. 2007 Oct 4;449(7162):570-5. Epub 2007 Sep 19. PMID:17882165 doi:10.1038/nature06155