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2r1x

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Revision as of 05:35, 8 March 2018

Crystal structure of S25-2 Fab in complex with Kdo analogues

Structural highlights

2r1x is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	1q9q, 2r1w, 2r1y, 2r23, 2r2b, 2r2e, 2r2h
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

To explore the molecular basis of antigen recognition by germline antibodies, we have determined to high resolution the structures of the near-germline monoclonal antibody S25-2 in complex with seven distinct carbohydrate antigens based on the bacterial sugar 3-deoxy-alpha-D-manno-oct-2-ulosonic acid (Kdo). In contrast to previous findings, the inherited germline Kdo monosaccharide binding site is not restricted to this bacterial sugar but is able to accommodate an array of substitutions and chemical modifications of Kdo, including naturally occurring antigens containing the related monosaccharide d-glycero-alpha-d-talo-oct-2-ulosonic acid as well as nonterminal Kdo residues. However, we show by surface plasmon resonance and ELISA how antibody S25-2 specificity is so dependent on the context in which the antigen is presented that a free disaccharide displays strong binding while the same lipid-A-bound disaccharide does not bind. These structures provide insight into how inherited germline genes code for immunoglobulins of limited flexibility that are capable of binding a range of epitopes from which affinity-matured antibodies are generated.

Exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes.,Brooks CL, Muller-Loennies S, Brade L, Kosma P, Hirama T, MacKenzie CR, Brade H, Evans SV J Mol Biol. 2008 Mar 21;377(2):450-68. Epub 2008 Jan 16. PMID:18272175^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Brooks CL, Muller-Loennies S, Brade L, Kosma P, Hirama T, MacKenzie CR, Brade H, Evans SV. Exploration of specificity in germline monoclonal antibody recognition of a range of natural and synthetic epitopes. J Mol Biol. 2008 Mar 21;377(2):450-68. Epub 2008 Jan 16. PMID:18272175 doi:S0022-2836(08)00036-3

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2r1x"

@@ Line 1: / Line 1: @@
 ==Crystal structure of S25-2 Fab in complex with Kdo analogues==
 <StructureSection load='2r1x' size='340' side='right' caption='[[2r1x]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KDA:(3-DEOXY-D-MANNO-OCT-2-ULOSONIC+ACID)-2-O-ALLYL'>KDA</scene>, <scene name='pdbligand=KDD:2,6-ANHYDRO-3,5-DIDEOXY-D-RIBO-OCT-2-ENONIC+ACID'>KDD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q9q|1q9q]], [[2r1w|2r1w]], [[2r1y|2r1y]], [[2r23|2r23]], [[2r2b|2r2b]], [[2r2e|2r2e]], [[2r2h|2r2h]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r1x OCA], [http://pdbe.org/2r1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r1x RCSB], [http://www.ebi.ac.uk/pdbsum/2r1x PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2r1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r1x OCA], [http://pdbe.org/2r1x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2r1x RCSB], [http://www.ebi.ac.uk/pdbsum/2r1x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2r1x ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 11: / Line 12: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r1x_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r1x_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 28: / Line 29: @@
 ==See Also==
-*[[Monoclonal Antibody|Monoclonal Antibody]]
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 == References ==
 <references/>

2r1x

From Proteopedia

Revision as of 05:35, 8 March 2018

Crystal structure of S25-2 Fab in complex with Kdo analogues

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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