This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2fe8
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2fe8 |SIZE=350|CAPTION= <scene name='initialview01'>2fe8</scene>, resolution 1.85Å | |PDB= 2fe8 |SIZE=350|CAPTION= <scene name='initialview01'>2fe8</scene>, resolution 1.85Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fe8 OCA], [http://www.ebi.ac.uk/pdbsum/2fe8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fe8 RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Ratia, K.]] | [[Category: Ratia, K.]] | ||
[[Category: Santarsiero, B D.]] | [[Category: Santarsiero, B D.]] | ||
| - | [[Category: BR]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: ZN]] | ||
[[Category: protease]] | [[Category: protease]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:01:37 2008'' |
Revision as of 00:01, 31 March 2008
| |||||||
| , resolution 1.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SARS coronavirus papain-like protease: structure of a viral deubiquitinating enzyme
Overview
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. In this work, we describe the 1.85-A crystal structure of the catalytic core of SARS-CoV PLpro and show that the overall architecture adopts a fold closely resembling that of known deubiquitinating enzymes. Key features, however, distinguish PLpro from characterized deubiquitinating enzymes, including an intact zinc-binding motif, an unobstructed catalytically competent active site, and the presence of an intriguing, ubiquitin-like N-terminal domain. To gain insight into the active-site recognition of the C-terminal tail of ubiquitin and the related LXGG motif, we propose a model of PLpro in complex with ubiquitin-aldehyde that reveals well defined sites within the catalytic cleft that help to account for strict substrate-recognition motifs.
About this Structure
2FE8 is a Single protein structure of sequence from Sars coronavirus. Full crystallographic information is available from OCA.
Reference
Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme., Ratia K, Saikatendu KS, Santarsiero BD, Barretto N, Baker SC, Stevens RC, Mesecar AD, Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):5717-22. Epub 2006 Mar 31. PMID:16581910
Page seeded by OCA on Mon Mar 31 03:01:37 2008
