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1sh4

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Revision as of 06:39, 8 March 2018

Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H

Structural highlights

1sh4 is a 1 chain structure with sequence from Bovin. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1nx7
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CYB5_BOVIN] Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

A comparative study on the solution structures of bovine microsomal cytochrome b5 (Tb5) and the mutant V45H has been achieved by 1D and 2D 1H-NMR spectroscopy to clarify the differences in the solution conformations between these two proteins. The results reveal that the global folding of the V45H mutant in solution is unchanged, but the subtle changes exist in the orientation of the axial ligand His39, and heme vinyl groups. The side chain of His45 in V45H mutant extends to the outer edge of the heme pocket leaving a cavity at the site originally occupied by the inner methyl group of Val45 residue. In addition, the imidazole ring of axial ligand His39 rotates counterclockwise by approximately 3 degrees around the His-Fe-His axis, and the 4-heme vinyl group turns to the space vacated by the removed side chain due to the mutation. Furthermore, the helix III of the heme pocket undergoes outward displacement, while the linkage between helix II and III is shifted leftward. These observations are not only consistent with the pattern of the pseudocontact shifts of the heme protons, but also well account for the lower stability of V45H mutant against heat and urea.

The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H.,Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX Protein Sci. 2004 Aug;13(8):2161-9. PMID:15273310^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang Q, Cao C, Wang ZQ, Wang YH, Wu H, Huang ZX. The comparative study on the solution structures of the oxidized bovine microsomal cytochrome b5 and mutant V45H. Protein Sci. 2004 Aug;13(8):2161-9. PMID:15273310 doi:10.1110/ps.04721104

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sh4"

@@ Line 1: / Line 1: @@
 ==Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H==
 <StructureSection load='1sh4' size='340' side='right' caption='[[1sh4]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nx7|1nx7]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sh4 OCA], [http://pdbe.org/1sh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sh4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sh4 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sh4 OCA], [http://pdbe.org/1sh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sh4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sh4 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1sh4_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1sh4_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1sh4

From Proteopedia

Revision as of 06:39, 8 March 2018

Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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