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2fid

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|PDB= 2fid |SIZE=350|CAPTION= <scene name='initialview01'>2fid</scene>, resolution 2.80&Aring;
|PDB= 2fid |SIZE=350|CAPTION= <scene name='initialview01'>2fid</scene>, resolution 2.80&Aring;
|SITE=
|SITE=
-
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= RABGEF1, RABEX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
|GENE= RABGEF1, RABEX5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
 +
|DOMAIN=
 +
|RELATEDENTRY=[[2fif|2FIF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fid OCA], [http://www.ebi.ac.uk/pdbsum/2fid PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fid RCSB]</span>
}}
}}
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[[Category: Hurley, J H.]]
[[Category: Hurley, J H.]]
[[Category: Lee, S.]]
[[Category: Lee, S.]]
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[[Category: ZN]]
 
[[Category: helix]]
[[Category: helix]]
[[Category: zinc finger]]
[[Category: zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:52:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:03:10 2008''

Revision as of 00:03, 31 March 2008

Image:2fid.gif


PDB ID 2fid

Drag the structure with the mouse to rotate
, resolution 2.80Å
Ligands:
Gene: RABGEF1, RABEX5 (Bos taurus)
Related: 2FIF


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin


Overview

Rabex-5 is an exchange factor for Rab5, a master regulator of endosomal trafficking. Rabex-5 binds monoubiquitin, undergoes covalent ubiquitination and contains an intrinsic ubiquitin ligase activity, all of which require an N-terminal A20 zinc finger followed immediately by a helix. The structure of the N-terminal portion of Rabex-5 bound to ubiquitin at 2.5-A resolution shows that Rabex-5-ubiquitin interactions occur at two sites. The first site is a new type of ubiquitin-binding domain, an inverted ubiquitin-interacting motif, which binds with approximately 29-microM affinity to the canonical Ile44 hydrophobic patch on ubiquitin. The second is a diaromatic patch on the A20 zinc finger, which binds with approximately 22-microM affinity to a polar region centered on Asp58 of ubiquitin. The A20 zinc-finger diaromatic patch mediates ubiquitin-ligase activity by directly recruiting a ubiquitin-loaded ubiquitin-conjugating enzyme.

About this Structure

2FID is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5., Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH, Nat Struct Mol Biol. 2006 Mar;13(3):264-71. Epub 2006 Feb 5. PMID:16462746

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