RecG protein
From Proteopedia
(New page: '''RecG''' The RecG protein of Escherichia coli is a Superfamily 2 (SF2) branched-DNA-specific helicase which promotes rescue of damaged replication forks by catalysing their unwinding ...) |
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| - | The RecG protein of Escherichia coli is a Superfamily 2 (SF2) branched-DNA-specific helicase which promotes rescue of damaged replication forks by catalysing their unwinding and conversion to Holliday junctions. A single RecG monomer unwinds two DNA duplexes; the leading strand duplex and the lagging strand duplex and then reanneal the parental and the newly synthetized strands. This mechanism of repair is ATP-dependent and does not require DNA double strand break. RecG works synergistically with the RuvABC repair system<ref>Singleton, M. R., Scaife, S. & Wigley, D. B. Structural analysis of DNA replication fork reversal by RecG. Cell 107, 79–89 (2001)</ref>. | + | The RecG protein of Escherichia coli is a Superfamily 2 (SF2) branched-DNA-specific helicase which promotes rescue of damaged replication forks by catalysing their unwinding and conversion to Holliday junctions. A single RecG monomer unwinds two DNA duplexes; the leading strand duplex and the lagging strand duplex and then reanneal the parental and the newly synthetized strands. This mechanism of repair is ATP-dependent and does not require DNA double strand break. RecG works synergistically with the RuvABC repair system<ref>Singleton, M. R., Scaife, S. & Wigley, D. B. Structural analysis of DNA replication fork reversal by RecG. Cell 107, 79–89 (2001)</ref>,<ref>Rudolph, C. J., Upton, A. L., Briggs, G. S. & Lloyd, R. G. Is RecG a general guardian of the bacterial genome? DNA Repair 9, 210–223 (2010)</ref>. |
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| + | '''SUPERFAMILY 2 HELICASES''' | ||
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| + | Helicases are a ubiquitous group of enzymes that use the energy of nucleoside triphosphate (NTP) hydrolysis to catalyze the separation of double-stranded nucleic acids (dsNA). Therefore, helicases are involved in essentially every step in DNA and RNA metabolism, including replication, DNA repair, recombination, transcription, translation, chromatin rearrangement, ribosome synthesis, RNA maturation and splicing, nuclear export, Holliday junction movement, and displacement of proteins from DNA and RNA. | ||
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| + | Superfamily 2 is the largest and most diverse of the helicase superfamilies. It has been further divided into families including RecQ-like, RecG-like, Rad3/XPD, Ski2-like, type I restriction enzyme, RIG-I-like, NS3/NPH-II, DEAH/RHA, DEAD-box, and Swi/Snf families based on sequence homology. Since SF2 helicases function in diverse parts of nucleic acid metabolism, defects are associated with a variety of diseases including predisposition to cancer, premature aging, immunodeficiency, and mental retardation<ref>Byrd, A. K., & Raney, K. D. (2012). Superfamily 2 helicases. Frontiers in Bioscience (Landmark Edition), 17, 2070–2088</ref>. | ||
'''Reference:'''<references /> | '''Reference:'''<references /> | ||
Revision as of 10:41, 29 March 2018
RecG
The RecG protein of Escherichia coli is a Superfamily 2 (SF2) branched-DNA-specific helicase which promotes rescue of damaged replication forks by catalysing their unwinding and conversion to Holliday junctions. A single RecG monomer unwinds two DNA duplexes; the leading strand duplex and the lagging strand duplex and then reanneal the parental and the newly synthetized strands. This mechanism of repair is ATP-dependent and does not require DNA double strand break. RecG works synergistically with the RuvABC repair system[1],[2].
SUPERFAMILY 2 HELICASES
Helicases are a ubiquitous group of enzymes that use the energy of nucleoside triphosphate (NTP) hydrolysis to catalyze the separation of double-stranded nucleic acids (dsNA). Therefore, helicases are involved in essentially every step in DNA and RNA metabolism, including replication, DNA repair, recombination, transcription, translation, chromatin rearrangement, ribosome synthesis, RNA maturation and splicing, nuclear export, Holliday junction movement, and displacement of proteins from DNA and RNA.
Superfamily 2 is the largest and most diverse of the helicase superfamilies. It has been further divided into families including RecQ-like, RecG-like, Rad3/XPD, Ski2-like, type I restriction enzyme, RIG-I-like, NS3/NPH-II, DEAH/RHA, DEAD-box, and Swi/Snf families based on sequence homology. Since SF2 helicases function in diverse parts of nucleic acid metabolism, defects are associated with a variety of diseases including predisposition to cancer, premature aging, immunodeficiency, and mental retardation[3].
Reference:- ↑ Singleton, M. R., Scaife, S. & Wigley, D. B. Structural analysis of DNA replication fork reversal by RecG. Cell 107, 79–89 (2001)
- ↑ Rudolph, C. J., Upton, A. L., Briggs, G. S. & Lloyd, R. G. Is RecG a general guardian of the bacterial genome? DNA Repair 9, 210–223 (2010)
- ↑ Byrd, A. K., & Raney, K. D. (2012). Superfamily 2 helicases. Frontiers in Bioscience (Landmark Edition), 17, 2070–2088
