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=== ''Msl-2'' ===
=== ''Msl-2'' ===
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The alternative splicing of ''msl-2'' is reliant on Sxl binding to both the 5' and 3' splice sites (Fig. 3). Sxl binds at the 3' splice site, replacing U2AF as in ''tra'' splicing. Sxl also competes with [http://www.uniprot.org/uniprot/Q26281 Rox8], which binds to the first intron. As a result, Sxl prevents splicing of the first intron of the ''msl-2'' primary transcript. Sxl also binds to the poly- U sequences of the 3' UTR to repress translation (Fig.3)<ref name="Panalva"/>doi:10.1128/mmbr.67.3.343-359.2003</ref>. When Sxl targets ''msl-2'', the first intron is retained<ref name="Black"/>. However, the retained intron is in the [https://en.wikipedia.org/wiki/Untranslated_region 5' UTR] and does not affect the reading frame<ref name="Black"/>.
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The alternative splicing of ''msl-2'' is reliant on Sxl binding to both the 5' and 3' splice sites (Fig. 3). Sxl binds at the 3' splice site, replacing U2AF as in ''tra'' splicing. Sxl also competes with [http://www.uniprot.org/uniprot/Q26281 Rox8], which binds to the first intron. As a result, Sxl prevents splicing of the first intron of the ''msl-2'' primary transcript. Sxl also binds to the poly- U sequences of the 3' UTR to repress translation (Fig.3)<ref name="Penalva"/>. When Sxl targets ''msl-2'', the first intron is retained<ref name="Black"/>. However, the retained intron is in the [https://en.wikipedia.org/wiki/Untranslated_region 5' UTR] and does not affect the reading frame<ref name="Black"/>.
=== Structural Basis for Recognition of Poly-U Sequences ===
=== Structural Basis for Recognition of Poly-U Sequences ===

Revision as of 18:26, 30 March 2018

Contents

Sex-Lethal Protein

Sex-Lethal protein

Drag the structure with the mouse to rotate

Additional Reading

For more information on the U2AF splicing factor.


Relevance

As Sxl functions as a splicing repressor, it may give insight into the effects of varying mechanisms of alternate splicing both in flies and other species. Sxl may also lead to understanding of human alternative splicing factors. As an RNA binding protein, research regarding Sxl may contribute to the understanding of enzymes with RNA recognition motifs. The Sxl RNP motif of RBD1 is also conserved in the ELAV family of proteins[1].

References

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 Handa N, Nureki O, Kurimoto K, Kim I, Sakamoto H, Shimura Y, Muto Y, Yokoyama S. Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein. Nature. 1999 Apr 15;398(6728):579-85. PMID:10217141 doi:10.1038/19242
  2. 2.0 2.1 2.2 2.3 Penalva LO, Sanchez L. RNA binding protein sex-lethal (Sxl) and control of Drosophila sex determination and dosage compensation. Microbiol Mol Biol Rev. 2003 Sep;67(3):343-59, table of contents. PMID:12966139
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 Black DL. Mechanisms of alternative pre-messenger RNA splicing. Annu Rev Biochem. 2003;72:291-336. doi: 10.1146/annurev.biochem.72.121801.161720., Epub 2003 Feb 27. PMID:12626338 doi:http://dx.doi.org/10.1146/annurev.biochem.72.121801.161720

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