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6fc6
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6fc6 is ON HOLD Authors: Kumar, A., Stangier, M.M., Steinmetz, M.O. Description: Bik1 CAP-Gly domain with ETF peptide from Bim1 [[Category: Unrelea...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Bik1 CAP-Gly domain with ETF peptide from Bim1== | |
| + | <StructureSection load='6fc6' size='340' side='right' caption='[[6fc6]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6fc6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FC6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fc6 OCA], [http://pdbe.org/6fc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fc6 RCSB], [http://www.ebi.ac.uk/pdbsum/6fc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fc6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/BIK1_YEAST BIK1_YEAST]] Required for nuclear fusion, chromosome disjunction, and nuclear segregation during mitosis. Probably required for the formation or stabilization of microtubules during mitosis and for spindle pole body fusion during conjugation. [[http://www.uniprot.org/uniprot/BIM1_YEAST BIM1_YEAST]] Binds microtubules. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In budding yeast, the microtubule plus-end tracking proteins Bik1 (CLIP-170) and Bim1 (EB1) form a complex that interacts with partners involved in spindle positioning, including Stu2 and Kar9. Here, we show that the CAP-Gly and coiled-coil domains of Bik1 interact with the C-terminal ETF peptide of Bim1 and the C-terminal tail region of Stu2, respectively. The crystal structures of the CAP-Gly domain of Bik1 (Bik1CG) alone and in complex with an ETF peptide revealed unique, functionally relevant CAP-Gly elements, establishing Bik1CG as a specific C-terminal phenylalanine recognition domain. Unlike the mammalian CLIP-170-EB1 complex, Bik1-Bim1 forms ternary complexes with the EB1-binding motifs SxIP and LxxPTPh, which are present in diverse proteins, including Kar9. Perturbation of the Bik1-Bim1 interaction in vivo affected Bik1 localization and astral microtubule length. Our results provide insight into the role of the Bik1-Bim1 interaction for cell division, and demonstrate that the CLIP-170-EB1 module is evolutionarily flexible. | ||
| - | + | Structure-Function Relationship of the Bik1-Bim1 Complex.,Stangier MM, Kumar A, Chen X, Farcas AM, Barral Y, Steinmetz MO Structure. 2018 Apr 3;26(4):607-618.e4. doi: 10.1016/j.str.2018.03.003. Epub 2018, Mar 22. PMID:29576319<ref>PMID:29576319</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 6fc6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Kumar, A]] | [[Category: Kumar, A]] | ||
| - | [[Category: | + | [[Category: Stangier, M M]] |
| - | [[Category: | + | [[Category: Steinmetz, M O]] |
| + | [[Category: Tip]] | ||
| + | [[Category: Bik1]] | ||
| + | [[Category: Bim1]] | ||
| + | [[Category: Cap-gly]] | ||
| + | [[Category: Cell cycle]] | ||
| + | [[Category: Etf]] | ||
| + | [[Category: Yeast]] | ||
Current revision
Bik1 CAP-Gly domain with ETF peptide from Bim1
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Categories: Kumar, A | Stangier, M M | Steinmetz, M O | Tip | Bik1 | Bim1 | Cap-gly | Cell cycle | Etf | Yeast
