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6f7a
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6f7a is ON HOLD Authors: Martiel, I., Zabara, A., Chong, J.Y.T., Stark, L., Cromer, B., Dummond, C.J., Mezzenga, R. Description: Gloeobacter Ligand...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Gloeobacter Ligand-gated Ion Channel (GLIC) closed state crystallized in an ultra-swollen lipidic mesophase== | |
| + | <StructureSection load='6f7a' size='340' side='right' caption='[[6f7a]], [[Resolution|resolution]] 6.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6f7a]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Glovi Glovi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F7A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F7A FirstGlance]. <br> | ||
| + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glvI, glr4197 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=251221 GLOVI])</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f7a OCA], [http://pdbe.org/6f7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f7a RCSB], [http://www.ebi.ac.uk/pdbsum/6f7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f7a ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/GLIC_GLOVI GLIC_GLOVI]] Cationic channel with similar permeabilities for Na(+) and K(+), that is activated by an increase of the proton concentration on the extracellular side. Displays no permeability for chloride ions. Shows slow kinetics of activation, no desensitization and a single channel conductance of 8 pS. Might contribute to adaptation to external pH change.<ref>PMID:17167423</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In meso crystallization of membrane proteins from lipidic mesophases is central to protein structural biology but limited to membrane proteins with small extracellular domains (ECDs), comparable to the water channels (3-5 nm) of the mesophase. Here we present a strategy expanding the scope of in meso crystallization to membrane proteins with very large ECDs. We combine monoacylglycerols and phospholipids to design thermodynamically stable ultra-swollen bicontinuous cubic phases of double-gyroid (Ia3d), double-diamond (Pn3m), and double-primitive (Im3m) space groups, with water channels five times larger than traditional lipidic mesophases, and showing re-entrant behavior upon increasing hydration, of sequences Ia3d-->Pn3m-->Ia3d and Pn3m-->Im3m-->Pn3m, unknown in lipid self-assembly. We use these mesophases to crystallize membrane proteins with ECDs inaccessible to conventional in meso crystallization, demonstrating the methodology on the Gloeobacter ligand-gated ion channel (GLIC) protein, and show substantial modulation of packing, molecular contacts and activation state of the ensued proteins crystals, illuminating a general strategy in protein structural biology. | ||
| - | + | Design of ultra-swollen lipidic mesophases for the crystallization of membrane proteins with large extracellular domains.,Zabara A, Chong JTY, Martiel I, Stark L, Cromer BA, Speziale C, Drummond CJ, Mezzenga R Nat Commun. 2018 Feb 7;9(1):544. doi: 10.1038/s41467-018-02996-5. PMID:29416037<ref>PMID:29416037</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Chong, J | + | <div class="pdbe-citations 6f7a" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Glovi]] | ||
| + | [[Category: Chong, J Y.T]] | ||
| + | [[Category: Cromer, B]] | ||
| + | [[Category: Dummond, C J]] | ||
[[Category: Martiel, I]] | [[Category: Martiel, I]] | ||
| - | [[Category: Stark, L]] | ||
[[Category: Mezzenga, R]] | [[Category: Mezzenga, R]] | ||
| - | [[Category: | + | [[Category: Stark, L]] |
| + | [[Category: Zabara, A]] | ||
| + | [[Category: Membrane protein]] | ||
| + | [[Category: Transport protein]] | ||
Current revision
Gloeobacter Ligand-gated Ion Channel (GLIC) closed state crystallized in an ultra-swollen lipidic mesophase
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