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| - | [[Image:1qy5.jpg|left|200px]] | + | #REDIRECT [[6d28]] This PDB entry is obsolete and replaced by 6d28 |
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| - | {{STRUCTURE_1qy5| PDB=1qy5 | SCENE= }}
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| - | '''Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA'''
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| - | ==Overview==
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| - | GRP94, the endoplasmic reticulum (ER) paralog of the chaperone Hsp90, plays an essential role in the structural maturation or secretion of a subset of proteins destined for transport to the cell surface, such as the Toll-like receptors 2 and 4, and IgG, respectively. GRP94 differs from cytoplasmic Hsp90 by exhibiting very weak ATP binding and hydrolysis activity. GRP94 also binds selectively to a series of substituted adenosine analogs. The high resolution crystal structures at 1.75-2.1 A of the N-terminal and adjacent charged domains of GRP94 in complex with N-ethylcarboxamidoadenosine, radicicol, and 2-chlorodideoxyadenosine reveals a structural mechanism for ligand discrimination among hsp90 family members. The structures also identify a putative subdomain that may act as a ligand-responsive switch. The residues of the charged region fold into a disordered loop whose termini are ordered and continue the twisted beta sheet that forms the structural core of the N-domain. This continuation of the beta sheet past the charged domain suggests a structural basis for the association of the N-terminal and middle domains of the full-length chaperone.
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| - | ==About this Structure==
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| - | 1QY5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QY5 OCA].
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| - | ==Reference==
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| - | Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation., Soldano KL, Jivan A, Nicchitta CV, Gewirth DT, J Biol Chem. 2003 Nov 28;278(48):48330-8. Epub 2003 Sep 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970348 12970348]
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| - | [[Category: Canis lupus familiaris]]
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| - | [[Category: Single protein]]
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| - | [[Category: Gewirth, D T.]]
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| - | [[Category: Jivan, A.]]
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| - | [[Category: Nicchitta, C V.]]
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| - | [[Category: Soldano, K L.]]
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| - | [[Category: Grp94]]
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| - | [[Category: Hsp90]]
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| - | [[Category: Neca]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:50:45 2008''
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