1wc5

From Proteopedia

Revision as of 15:26, 5 November 2007

SOLUBLE ADENYLYL CYCLASE CYAC FROM S. PLATENSIS IN COMPLEX WITH ALPHA,BETA-METHYLENE-ATP IN PRESENCE OF BICARBONATE

Overview

In an evolutionarily conserved signaling pathway, 'soluble' adenylyl, cyclases (sACs) synthesize the ubiquitous second messenger cyclic, adenosine 3',5'-monophosphate (cAMP) in response to bicarbonate and, calcium signals. Here, we present crystal structures of a cyanobacterial, sAC enzyme in complex with ATP analogs, calcium and bicarbonate, which, represent distinct catalytic states of the enzyme. The structures reveal, that calcium occupies the first ion-binding site and directly mediates, nucleotide binding. The single ion-occupied, nucleotide-bound state, defines a novel, open adenylyl cyclase state. In contrast, bicarbonate, increases the catalytic rate by inducing marked active site closure and, recruiting a second, catalytic ion. The phosphates of the bound substrate, analogs are rearranged, which would facilitate product formation and, release. The mechanisms of calcium and bicarbonate sensing define a, reaction pathway involving active site closure and metal recruitment that, may be universal for class III cyclases.

About this Structure

1WC5 is a Single protein structure of sequence from Arthrospira platensis with MG, CA and APC as ligands. Active as Adenylate cyclase, with EC number 4.6.1.1 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

Bicarbonate activation of adenylyl cyclase via promotion of catalytic active site closure and metal recruitment., Steegborn C, Litvin TN, Levin LR, Buck J, Wu H, Nat Struct Mol Biol. 2005 Jan;12(1):32-7. Epub 2004 Dec 26. PMID:15619637

Page seeded by OCA on Mon Nov 5 17:32:00 2007