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2jex

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|ACTIVITY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jex OCA], [http://www.ebi.ac.uk/pdbsum/2jex PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jex RCSB]</span>
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[[Category: viral transcription factor]]
[[Category: viral transcription factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:41:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:57:11 2008''

Revision as of 00:57, 31 March 2008

Image:2jex.jpg


PDB ID 2jex

Drag the structure with the mouse to rotate
, resolution 2.35Å
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



TRANSCRIPTION ACTIVATOR STRUCTURE REVEALS REDOX CONTROL OF A REPLICATION INITIATION REACTION


Overview

Redox changes are one of the factors that influence cell-cycle progression and that control the processes of cellular proliferation, differentiation, senescence and apoptosis. Proteins regulated through redox-sensitive cysteines have been characterized but specific 'sulphydryl switches' in replication proteins remain to be identified. In bovine papillomavirus type-1, DNA replication begins when the viral transcription factor E2 recruits the viral initiator protein E1 to the origin of DNA replication (ori). Here we show that a novel dimerization interface in the E2 transcription activation domain is stabilized by a disulphide bond. Oxidative cross-linking via Cys57 sequesters the interaction surface between E1 and E2, preventing pre-initiation and replication initiation complex formation. Our data demonstrate that as well as a mechanism for regulating DNA binding, redox reactions can control replication by modulating the tertiary structure of critical protein factors using a specific redox sensor.

About this Structure

2JEX is a Single protein structure of sequence from Bovine papillomavirus type 1. Full crystallographic information is available from OCA.

Reference

Transcription activator structure reveals redox control of a replication initiation reaction., Sanders CM, Sizov D, Seavers PR, Ortiz-Lombardia M, Antson AA, Nucleic Acids Res. 2007;35(10):3504-15. Epub 2007 May 3. PMID:17478495

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