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2jgp
From Proteopedia
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|PDB= 2jgp |SIZE=350|CAPTION= <scene name='initialview01'>2jgp</scene>, resolution 1.85Å | |PDB= 2jgp |SIZE=350|CAPTION= <scene name='initialview01'>2jgp</scene>, resolution 1.85Å | ||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Dio+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Dio+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Chain+A'>AC5</scene> | |SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:So4+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Dio+Binding+Site+For+Chain+A'>AC3</scene>, <scene name='pdbsite=AC4:Dio+Binding+Site+For+Chain+A'>AC4</scene> and <scene name='pdbsite=AC5:Na+Binding+Site+For+Chain+A'>AC5</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1dny|1DNY]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jgp OCA], [http://www.ebi.ac.uk/pdbsum/2jgp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jgp RCSB]</span> | ||
}} | }} | ||
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[[Category: Samel, S A.]] | [[Category: Samel, S A.]] | ||
[[Category: Schoenafinger, G.]] | [[Category: Schoenafinger, G.]] | ||
| - | [[Category: DIO]] | ||
| - | [[Category: NA]] | ||
| - | [[Category: SO4]] | ||
[[Category: antibiotic biosynthesis]] | [[Category: antibiotic biosynthesis]] | ||
[[Category: antibiotic]] | [[Category: antibiotic]] | ||
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[[Category: tyrocidine]] | [[Category: tyrocidine]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:57:59 2008'' |
Revision as of 00:58, 31 March 2008
| |||||||
| , resolution 1.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , and | ||||||
| Ligands: | , , | ||||||
| Related: | 1DNY
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE TYCC5-6 PCP-C BIDOMAIN OF THE TYROCIDINE SYNTHETASE TYCC
Overview
The crystal structure of the bidomain PCP-C from modules 5 and 6 of the nonribosomal tyrocidine synthetase TycC was determined at 1.8 A resolution. The bidomain structure reveals a V-shaped condensation domain, the canyon-like active site groove of which is associated with the preceding peptidyl carrier protein (PCP) domain at its donor side. The relative arrangement of the PCP and the peptide bond-forming condensation (C) domain places the active sites approximately 50 A apart. Accordingly, this PCP-C structure represents a conformational state prior to peptide transfer from the donor-PCP to the acceptor-PCP domain, implying the existence of additional states of PCP-C domain interaction during catalysis. Additionally, PCP-C exerts a mode of cyclization activity that mimics peptide bond formation catalyzed by C domains. Based on mutational data and pK value analysis of active site residues, it is suggested that nonribosomal peptide bond formation depends on electrostatic interactions rather than on general acid/base catalysis.
About this Structure
2JGP is a Single protein structure of sequence from Brevibacillus brevis. Full crystallographic information is available from OCA.
Reference
Structural and functional insights into a peptide bond-forming bidomain from a nonribosomal peptide synthetase., Samel SA, Schoenafinger G, Knappe TA, Marahiel MA, Essen LO, Structure. 2007 Jul;15(7):781-92. PMID:17637339
Page seeded by OCA on Mon Mar 31 03:57:59 2008
Categories: Brevibacillus brevis | Single protein | Essen, L O. | Knappe, T A. | Marahiel, M A. | Samel, S A. | Schoenafinger, G. | Antibiotic biosynthesis | Antibiotic | Condensation domain | Ligase | Multifunctional enzyme | Nonribosomal peptide synthetase | Peptide bond formation | Peptidyl carrier domain | Phosphopantetheine | Tyrocidine
