2jxm
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2jxm |SIZE=350|CAPTION= <scene name='initialview01'>2jxm</scene> | |PDB= 2jxm |SIZE=350|CAPTION= <scene name='initialview01'>2jxm</scene> | ||
|SITE= <scene name='pdbsite=AC1:Cu+Binding+Site+For+Residue+A+98'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Cu+Binding+Site+For+Residue+A+98'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene> | + | |LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]), petA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]) | |GENE= petE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]), petA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223 Prochlorothrix hollandica]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1b3i|1B3I]], [[1ci3|1CI3]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jxm OCA], [http://www.ebi.ac.uk/pdbsum/2jxm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jxm RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Hulsker, R.]] | [[Category: Hulsker, R.]] | ||
[[Category: Ubbink, M.]] | [[Category: Ubbink, M.]] | ||
| - | [[Category: CU]] | ||
| - | [[Category: HEC]] | ||
[[Category: copper]] | [[Category: copper]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
| Line 33: | Line 34: | ||
[[Category: transport]] | [[Category: transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:02:23 2008'' |
Revision as of 01:02, 31 March 2008
| |||||||
| Sites: | |||||||
| Ligands: | , | ||||||
| Gene: | petE (Prochlorothrix hollandica), petA (Prochlorothrix hollandica) | ||||||
| Related: | 1B3I, 1CI3
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ensemble of twenty structures of the Prochlorothrix hollandica plastocyanin- cytochrome f complex
Overview
The nature of transient protein complexes can range from a highly dynamic ensemble of orientations to a single well-defined state. This represents variation in the equilibrium between the encounter and final, functional state. The transient complex between plastocyanin (Pc) and cytochrome f (cytf) of the cyanobacterium Prochlorothrix hollandica was characterized by NMR spectroscopy. Intermolecular pseudocontact shifts and chemical shift perturbations were used as restraints in docking calculations to determine the structure of the wild-type Pc-cytf complex. The orientation of Pc is similar to orientations found in Pc-cytf complexes from other sources. Electrostatics seems to play a modest role in complex formation. A large variability in the ensemble of lowest energy structures indicates a dynamic nature of the complex. Two unusual hydrophobic patch residues in Pc have been mutated to the residues found in other plastocyanins (Y12G/P14L). The binding constants are similar for the complexes of cytf with wild-type Pc and mutant Pc, but the chemical shift perturbations are smaller for the complex with mutant Pc. Docking calculations for the Y12G/P14L Pc-cytf complex did not produce a converged ensemble of structures. Simulations of the dynamics were performed using the observed averaged NMR parameters as input. The results indicate a surprisingly large amplitude of mobility of Y12G/P14L Pc within the complex. It is concluded that the double mutation shifts the complex further from the well-defined toward the encounter state.
About this Structure
2JXM is a Protein complex structure of sequences from Prochlorothrix hollandica. Full crystallographic information is available from OCA.
Reference
Dynamics in the transient complex of plastocyanin-cytochrome f from Prochlorothrix hollandica., Hulsker R, Baranova MV, Bullerjahn GS, Ubbink M, J Am Chem Soc. 2008 Feb 13;130(6):1985-91. Epub 2008 Jan 18. PMID:18201089
Page seeded by OCA on Mon Mar 31 04:02:23 2008
