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2mjn

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Revision as of 22:38, 9 August 2018

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1

Structural highlights

2mjn is a 1 chain structure with sequence from Human. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	TIA1 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TIA1_HUMAN] Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

Publication Abstract from PubMed

Alternative pre-messenger ribonucleic acid (pre-mRNA) splicing is an essential process in eukaryotic gene regulation. The T-cell intracellular antigen-1 (TIA-1) is an apoptosis-promoting factor that modulates alternative splicing of transcripts, including the pre-mRNA encoding the membrane receptor Fas. TIA-1 is a multi-domain ribonucleic acid (RNA) binding protein that recognizes poly-uridine tract RNA sequences to facilitate 5' splice site recognition by the U1 small nuclear ribonucleoprotein (snRNP). Here, we characterize the RNA interaction and conformational dynamics of TIA-1 by nuclear magnetic resonance (NMR), isothermal titration calorimetry (ITC) and small angle X-ray scattering (SAXS). Our NMR-derived solution structure of TIA-1 RRM2-RRM3 (RRM2,3) reveals that RRM2 adopts a canonical RNA recognition motif (RRM) fold, while RRM3 is preceded by an non-canonical helix alpha0. NMR and SAXS data show that all three RRMs are largely independent structural modules in the absence of RNA, while RNA binding induces a compact arrangement. RRM2,3 binds to pyrimidine-rich FAS pre-mRNA or poly-uridine (U9) RNA with nanomolar affinities. RRM1 has little intrinsic RNA binding affinity and does not strongly contribute to RNA binding in the context of RRM1,2,3. Our data unravel the role of binding avidity and the contributions of the TIA-1 RRMs for recognition of pyrimidine-rich RNAs.

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1.,Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M Nucleic Acids Res. 2014 Mar 25. PMID:24682828^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang I, Hennig J, Jagtap PK, Sonntag M, Valcarcel J, Sattler M. Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1. Nucleic Acids Res. 2014 Mar 25. PMID:24682828 doi:http://dx.doi.org/10.1093/nar/gku193

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mjn"

@@ Line 1: / Line 1: @@
 ==Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1==
 <StructureSection load='2mjn' size='340' side='right' caption='[[2mjn]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2mjn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJN FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TIA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjn OCA], [http://pdbe.org/2mjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjn RCSB], [http://www.ebi.ac.uk/pdbsum/2mjn PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjn OCA], [http://pdbe.org/2mjn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjn RCSB], [http://www.ebi.ac.uk/pdbsum/2mjn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjn ProSAT]</span></td></tr>
 </table>
 == Function ==

2mjn

From Proteopedia

Revision as of 22:38, 9 August 2018

Structure, dynamics and RNA binding of the multi-domain splicing factor TIA-1

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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