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6h1k
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The major G-quadruplex form of HIV-1 LTR== |
| + | <StructureSection load='6h1k' size='340' side='right' caption='[[6h1k]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6h1k]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H1K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6H1K FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6h1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h1k OCA], [http://pdbe.org/6h1k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h1k RCSB], [http://www.ebi.ac.uk/pdbsum/6h1k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h1k ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Nucleic acids can form noncanonical four-stranded structures called G-quadruplexes. G-quadruplex-forming sequences are found in several genomes including human and viruses. Previous studies showed that the G-rich sequence located in the U3 promoter region of the HIV-1 long terminal repeat (LTR) folds into a set of dynamically interchangeable G-quadruplex structures. G-quadruplexes formed in the LTR could act as silencer elements to regulate viral transcription. Stabilization of LTR G-quadruplexes by G-quadruplex-specific ligands resulted in decreased viral production, suggesting the possibility of targeting viral G-quadruplex structures for antiviral purposes. Among all the G-quadruplexes formed in the LTR sequence, LTR-III was shown to be the major G-quadruplex conformation in vitro. Here we report the NMR structure of LTR-III in K(+) solution, revealing the formation of a unique quadruplex-duplex hybrid consisting of a three-layer (3 + 1) G-quadruplex scaffold, a 12-nt diagonal loop containing a conserved duplex-stem, a 3-nt lateral loop, a 1-nt propeller loop, and a V-shaped loop. Our structure showed several distinct features including a quadruplex-duplex junction, representing an attractive motif for drug targeting. The structure solved in this study may be used as a promising target to selectively impair the viral cycle. | ||
| - | + | Major G-Quadruplex Form of HIV-1 LTR Reveals a (3 + 1) Folding Topology Containing a Stem-Loop.,Butovskaya E, Heddi B, Bakalar B, Richter SN, Phan AT J Am Chem Soc. 2018 Oct 9. doi: 10.1021/jacs.8b05332. PMID:30299955<ref>PMID:30299955</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6h1k" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bakalar, B]] | ||
| + | [[Category: Butovskaya, E]] | ||
| + | [[Category: Heddi, B]] | ||
| + | [[Category: Phan, A T]] | ||
| + | [[Category: Richter, S N]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: G-quadruplex structure]] | ||
| + | [[Category: Hiv-1 ltr]] | ||
| + | [[Category: Stem loop]] | ||
Revision as of 08:10, 17 October 2018
The major G-quadruplex form of HIV-1 LTR
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