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2z4h

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Revision as of 09:35, 17 October 2018

Crystal structure of the Cpx pathway activator NlpE from Escherichia coli

Structural highlights

2z4h is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	2z4i
Gene:	nlpE ("Bacillus coli" Migula 1895)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NLPE_ECOLI] Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

NlpE, an outer membrane lipoprotein, functions during envelope stress responses in Gram-negative bacteria. In Escherichia coli, adhesion to abiotic surfaces has been reported to activate the Cpx pathway in an NlpE-dependent manner. External copper ions are also thought to activate the Cpx pathway mediated by NlpE. We determined the crystal structure of NlpE from E. coli at 2.6 A resolution. The structure showed that NlpE consists of two beta barrel domains. The N-terminal domain resembles the bacterial lipocalin Blc, and the C-terminal domain has an oligonucleotide/oligosaccharide-binding (OB) fold. From both biochemical analyses and the crystal structure, it can be deduced that the cysteine residues in the CXXC motif may be chemically active. Furthermore, two monomers in the asymmetric unit form an unusual 3D domain-swapped dimer. These findings indicate that tertiary and/or quaternary structural instability may be responsible for Cpx pathway activation.

Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli.,Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K Structure. 2007 Aug;15(8):963-76. PMID:17698001^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Miyadai H, Tanaka-Masuda K, Matsuyama S, Tokuda H. Effects of lipoprotein overproduction on the induction of DegP (HtrA) involved in quality control in the Escherichia coli periplasm. J Biol Chem. 2004 Sep 17;279(38):39807-13. Epub 2004 Jul 13. PMID:15252048 doi:http://dx.doi.org/10.1074/jbc.M406390200
↑ Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K. Structural studies of the Cpx pathway activator NlpE on the outer membrane of Escherichia coli. Structure. 2007 Aug;15(8):963-76. PMID:17698001 doi:10.1016/j.str.2007.06.014

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z4h"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the Cpx pathway activator NlpE from Escherichia coli==
 <StructureSection load='2z4h' size='340' side='right' caption='[[2z4h]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2z4i|2z4i]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nlpE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4h OCA], [http://pdbe.org/2z4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z4h RCSB], [http://www.ebi.ac.uk/pdbsum/2z4h PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z4h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z4h OCA], [http://pdbe.org/2z4h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2z4h RCSB], [http://www.ebi.ac.uk/pdbsum/2z4h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2z4h ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4h_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z4h_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2z4h

From Proteopedia

Revision as of 09:35, 17 October 2018

Crystal structure of the Cpx pathway activator NlpE from Escherichia coli

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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