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6gup

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Siderophore hydrolase EstB from Aspergillus fumigatus

Structural highlights

6gup is a 2 chain structure with sequence from Aspfm. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	AFUA_3G03390 (ASPFM)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[SIDJ_ASPFU] Displays specific fusarinine C (FsC) esterase activity but does not hydrolyze triacetylfusarinine C (TAFC), which has the same core structure as fusarinine C (PubMed:24038704). Both extra- and intracellular siderophores have been shown to be crucial for the virulence (PubMed:24038704). Subsequent to chelation of iron and uptake, FsC and TAFC are hydrolyzed and the iron is transferred to the metabolism or to the intracellular siderophore ferricrocin (FC) for transport and storage of iron (PubMed:24038704).^[1]

Publication Abstract from PubMed

Fungi utilize high-affinity chelators termed siderophores with chemically diverse structures to scavenge the essential nutrient iron from their surroundings. As they are among the strongest known Fe3+ binding agents, intracellular release of the heavy metal atom is facilitated by the activity of specific hydrolases. In this work, we report the characterization and x-ray structures of four siderophore esterases, AfEstB and AfSidJ from Aspergillus fumigatus, as well as AnEstB and AnEstA from Aspergillus nidulans. Even though they all display the conserved alpha/beta-hydrolase fold, we found remarkable structural and enzymatic discrepancies in their adaption to both related and chemically diverse substrates. A complex structure of AfEstB and its substrate triacetylfusarinine C gives insights into an active enzyme, with a tetrahedral coordination between the catalytic serine and the scissile ester bond.

Iron scavenging in Aspergillus species: Structural and biochemical insights into fungal siderophore esterases.,Ecker F, Haas H, Groll M, Huber E Angew Chem Int Ed Engl. 2018 Aug 1. doi: 10.1002/anie.201807093. PMID:30070018^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grundlinger M, Gsaller F, Schrettl M, Lindner H, Haas H. Aspergillus fumigatus SidJ mediates intracellular siderophore hydrolysis. Appl Environ Microbiol. 2013 Dec;79(23):7534-6. doi: 10.1128/AEM.01285-13. Epub, 2013 Sep 13. PMID:24038704 doi:http://dx.doi.org/10.1128/AEM.01285-13
↑ Ecker F, Haas H, Groll M, Huber E. Iron scavenging in Aspergillus species: Structural and biochemical insights into fungal siderophore esterases. Angew Chem Int Ed Engl. 2018 Aug 1. doi: 10.1002/anie.201807093. PMID:30070018 doi:http://dx.doi.org/10.1002/anie.201807093

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6gup"

@@ Line 3: / Line 3: @@
 <StructureSection load='6gup' size='340' side='right' caption='[[6gup]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6gup]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GUP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6gup]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfm Aspfm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GUP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GUP FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_3G03390 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=746128 ASPFM])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gup FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gup OCA], [http://pdbe.org/6gup PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gup RCSB], [http://www.ebi.ac.uk/pdbsum/6gup PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gup ProSAT]</span></td></tr>
 </table>
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 6gup" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Lipase|Lipase]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Aspfm]]
 [[Category: Ecker, F]]
 [[Category: Groll, M]]

6gup

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Current revision

Siderophore hydrolase EstB from Aspergillus fumigatus

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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