2-oxoisovalerate dehydrogenase
From Proteopedia
(Difference between revisions)
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| - | <StructureSection load=' | + | <StructureSection load='1x80' size='340' side='right' caption='Caption for this structure' scene=''> |
== Function == | == Function == | ||
| - | '''2-oxoisovalerate dehydrogenase''' (OIVD) or '''branched-chain α-keto acid decarboxylase''' is part of a multienzyme complex metabolizing pyruvate, 2-oxogluterate and branched-chain 2-oxo acids. The multienzyme complex contains OIVD, lipoamide acyltransferase and lipoamide dehydrogenase. The active site of OIVD contains the cofactor thiamin diphosphate ( | + | '''2-oxoisovalerate dehydrogenase''' (OIVD) or '''branched-chain α-keto acid decarboxylase''' is part of a multienzyme complex metabolizing pyruvate, 2-oxogluterate and branched-chain 2-oxo acids. The multienzyme complex contains three catalytic components: OIVD, lipoamide acyltransferase and lipoamide dehydrogenase, a kinase and a phosphatase. The active site of OIVD contains the cofactor thiamin diphosphate (ThDP)<ref>PMID:10426958</ref>. |
== Disease == | == Disease == | ||
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== Structural highlights == | == Structural highlights == | ||
| + | The structure of the phosphorylated human OIVD shows the cofactor ThDP interacting with residues from both α and β subunits and the phosphorylated Ser292 residue. Phosphorylation of Ser292 of the α subunit interferes with ThDP binding<ref>PMID:15576032</ref>. | ||
</StructureSection> | </StructureSection> | ||
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[[2bp7]] - OIVD α+β subunits - ''Pseudomonas putida''<br /> | [[2bp7]] - OIVD α+β subunits - ''Pseudomonas putida''<br /> | ||
[[1v1r]] - hOIVD α+β subunits - human<br /> | [[1v1r]] - hOIVD α+β subunits - human<br /> | ||
| - | [[1u5b]], [[1x80]], [[1ols]] - hOIVD α+β subunits + | + | [[1u5b]], [[1x80]], [[1ols]] - hOIVD α+β subunits + ThDP <br /> |
| - | [[1x7x]], [[1x7w]], [[1x7y]], [[1x7z]], [[2j9f]], [[1olu]], [[2bfb]], [[2bfd]], [[2bfe]], [[1v11]] - hOIVD α (mutant)+ β subunits + | + | [[1x7x]], [[1x7w]], [[1x7y]], [[1x7z]], [[2j9f]], [[1olu]], [[2bfb]], [[2bfd]], [[2bfe]], [[1v11]] - hOIVD α (mutant)+ β subunits + ThDP derivative<br /> |
| - | [[1olx]] - hOIVD α+β (mutant) subunits + | + | [[1olx]] - hOIVD α+β (mutant) subunits + ThDP<br /> |
[[1wci]] - hOIVD α+β subunits + thiamine derivative<br /> | [[1wci]] - hOIVD α+β subunits + thiamine derivative<br /> | ||
[[2bfe]] - hOIVD α+β subunits + pyrimidine derivative<br /> | [[2bfe]] - hOIVD α+β subunits + pyrimidine derivative<br /> | ||
[[2bfc]] - hOIVD α (mutant)+ β subunits + pyrimidine derivative<br /> | [[2bfc]] - hOIVD α (mutant)+ β subunits + pyrimidine derivative<br /> | ||
| - | [[1v16]] - hOIVD α+β subunits + | + | [[1v16]] - hOIVD α+β subunits + ThDP + benzamidine<br /> |
| - | [[1v1m]] - hOIVD α (mutant)+ β subunits + | + | [[1v1m]] - hOIVD α (mutant)+ β subunits + ThDP + benzamidine<br /> |
| - | [[2bew]], [[2beu]], [[2bev]] - hOIVD α+β subunits + | + | [[2bew]], [[2beu]], [[2bev]] - hOIVD α+β subunits + ThDP derivative + peptide<br /> |
== References == | == References == | ||
Revision as of 09:38, 6 November 2018
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3D structures of 2-oxoisovalerate dehydrogenase
Updated on 06-November-2018
2bp7 - OIVD α+β subunits - Pseudomonas putida
1v1r - hOIVD α+β subunits - human
1u5b, 1x80, 1ols - hOIVD α+β subunits + ThDP
1x7x, 1x7w, 1x7y, 1x7z, 2j9f, 1olu, 2bfb, 2bfd, 2bfe, 1v11 - hOIVD α (mutant)+ β subunits + ThDP derivative
1olx - hOIVD α+β (mutant) subunits + ThDP
1wci - hOIVD α+β subunits + thiamine derivative
2bfe - hOIVD α+β subunits + pyrimidine derivative
2bfc - hOIVD α (mutant)+ β subunits + pyrimidine derivative
1v16 - hOIVD α+β subunits + ThDP + benzamidine
1v1m - hOIVD α (mutant)+ β subunits + ThDP + benzamidine
2bew, 2beu, 2bev - hOIVD α+β subunits + ThDP derivative + peptide
References
- ↑ Aevarsson A, Seger K, Turley S, Sokatch JR, Hol WG. Crystal structure of 2-oxoisovalerate and dehydrogenase and the architecture of 2-oxo acid dehydrogenase multienzyme complexes. Nat Struct Biol. 1999 Aug;6(8):785-92. PMID:10426958 doi:10.1038/11563
- ↑ Wynn RM, Kato M, Machius M, Chuang JL, Li J, Tomchick DR, Chuang DT. Molecular mechanism for regulation of the human mitochondrial branched-chain alpha-ketoacid dehydrogenase complex by phosphorylation. Structure. 2004 Dec;12(12):2185-96. PMID:15576032 doi:10.1016/j.str.2004.09.013
