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3ckv

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Revision as of 13:55, 7 November 2018

Crystal Structure of a Mycobacterial Protein

Structural highlights

3ckv is a 1 chain structure with sequence from Mycobacterium paratuberculosis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	3ckj, 3ckn, 3kco, 3kcq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GPGS_MYCPA] Involved in the biosynthesis of methylglucose lipopolysaccharides (MGLPs). Catalyzes the condensation of UDP-glucose and 3-phospho-glycerate (3-PGA) to yield glucosyl-3-phosphoglycerate (GPG). Although UDP-glucose is the natural donor sugar, GpgS can also use UDP-GalNAc, UDPGlcNAc, UDP-alpha-L-arabinose, UDP-Gal, UDP-Glc, UDP-alpha-Dglucuronic acid, and UDP-alpha-D-xylose as donor substrates.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Glycosyltransferases (GTs) are a large and ubiquitous family of enzymes that specifically transfer sugar moieties to a range of substrates. Mycobacterium tuberculosis contains a large number of GTs, many of which are implicated in cell wall synthesis, yet the majority of these GTs remain poorly characterized. Here, we report the high resolution crystal structures of an essential GT (MAP2569c) from Mycobacterium avium subsp. paratuberculosis (a close homologue of Rv1208 from M. tuberculosis) in its apo- and ligand-bound forms. The structure adopted the GT-A fold and possessed the characteristic DXD motif that coordinated an Mn(2+) ion. Atypical of most GTs characterized to date, MAP2569c exhibited specificity toward the donor substrate, UDP-glucose. The structure of this ligated complex revealed an induced fit binding mechanism and provided a basis for this unique specificity. Collectively, the structural features suggested that MAP2569c may adopt a "retaining" enzymatic mechanism, which has implications for the classification of other GTs in this large superfamily.

Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species.,Fulton Z, McAlister A, Wilce MC, Brammananth R, Zaker-Tabrizi L, Perugini MA, Bottomley SP, Coppel RL, Crellin PK, Rossjohn J, Beddoe T J Biol Chem. 2008 Oct 10;283(41):27881-90. Epub 2008 Jul 30. PMID:18667419^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fulton Z, McAlister A, Wilce MC, Brammananth R, Zaker-Tabrizi L, Perugini MA, Bottomley SP, Coppel RL, Crellin PK, Rossjohn J, Beddoe T. Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species. J Biol Chem. 2008 Oct 10;283(41):27881-90. Epub 2008 Jul 30. PMID:18667419 doi:10.1074/jbc.M801853200
↑ Fulton Z, McAlister A, Wilce MC, Brammananth R, Zaker-Tabrizi L, Perugini MA, Bottomley SP, Coppel RL, Crellin PK, Rossjohn J, Beddoe T. Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species. J Biol Chem. 2008 Oct 10;283(41):27881-90. Epub 2008 Jul 30. PMID:18667419 doi:10.1074/jbc.M801853200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ckv"

Categories: Mycobacterium paratuberculosis | Marland, Z | Rossjohn, J | Mycobacteria | Unknown function

@@ Line 1: / Line 1: @@
 ==Crystal Structure of a Mycobacterial Protein==
 <StructureSection load='3ckv' size='340' side='right' caption='[[3ckv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ckv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_avium_subsp._paratuberculosis Mycobacterium avium subsp. paratuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CKV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ckv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_paratuberculosis Mycobacterium paratuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CKV FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ckj|3ckj]], [[3ckn|3ckn]], [[3kco|3kco]], [[3kcq|3kcq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ckv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ckv OCA], [http://pdbe.org/3ckv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ckv RCSB], [http://www.ebi.ac.uk/pdbsum/3ckv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ckv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ckv OCA], [http://pdbe.org/3ckv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ckv RCSB], [http://www.ebi.ac.uk/pdbsum/3ckv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ckv ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/3ckv_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ck/3ckv_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Mycobacterium avium subsp. paratuberculosis]]
+[[Category: Mycobacterium paratuberculosis]]
 [[Category: Marland, Z]]
 [[Category: Rossjohn, J]]
 [[Category: Mycobacteria]]
 [[Category: Unknown function]]

3ckv

From Proteopedia

Revision as of 13:55, 7 November 2018

Crystal Structure of a Mycobacterial Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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