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6ghg

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'''Unreleased structure'''
 
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The entry 6ghg is ON HOLD
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==Variable heavy - variable light domain and Fab-arm CrossMabs with charged residue exchanges==
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<StructureSection load='6ghg' size='340' side='right' caption='[[6ghg]], [[Resolution|resolution]] 1.88&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6ghg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GHG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GHG FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ghg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ghg OCA], [http://pdbe.org/6ghg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ghg RCSB], [http://www.ebi.ac.uk/pdbsum/6ghg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ghg ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Technologies for the production of bispecific antibodies need to overcome two major challenges. The first one is correct heavy chain assembly, which was solved by knobs-into-holes technology or charge interactions in the CH3 domains. The second challenge is correct light chain assembly. This can be solved by engineering the Fab-arm interfaces or applying the immunoglobulin domain crossover approach. There are three different crossovers possible, namely Fab-arm, constant domain and variable domain crossovers. The CrossMabCH1-CL exchange does not lead to the formation of unexpected side products, whereas the CrossMabFab and the CrossMabVH-VL formats result in the formation of typical side products. Thus, CrossMabCH1-CL was initially favored for therapeutic antibody development. Here, we report a novel improved CrossMab design principle making use of site-specific positional exchanges of charged amino acid pairs in the constant domain of these CrossMabs to enable the correct light chain assembly in the CrossMabVH-VL and improvements for the CrossMabFab design.
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Authors: Regula, J., Imhof-Jung, S., Molhoj, M., Benz, J., Ehler, A., Bujotzek, A., Schaefer, W., Klein, C.
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Variable heavy-variable light domain and Fab-arm CrossMabs with charged residue exchanges to enforce correct light chain assembly.,Regula JT, Imhof-Jung S, Molhoj M, Benz J, Ehler A, Bujotzek A, Schaefer W, Klein C Protein Eng Des Sel. 2018 Aug 28. pii: 5085589. doi: 10.1093/protein/gzy021. PMID:30169707<ref>PMID:30169707</ref>
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Description: Variable heavy -variable light domain and Fab-arm CrossMabs with charged residue exchanges
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 6ghg" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Human]]
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[[Category: Benz, J]]
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[[Category: Bujotzek, A]]
[[Category: Ehler, A]]
[[Category: Ehler, A]]
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[[Category: Schaefer, W]]
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[[Category: Imhof-Jung, S]]
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[[Category: Klein, C]]
[[Category: Molhoj, M]]
[[Category: Molhoj, M]]
[[Category: Regula, J]]
[[Category: Regula, J]]
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[[Category: Klein, C]]
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[[Category: Schaefer, W]]
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[[Category: Benz, J]]
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[[Category: Ang2]]
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[[Category: Imhof-Jung, S]]
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[[Category: Antibody]]
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[[Category: Bujotzek, A]]
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[[Category: Charge variant]]
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[[Category: Crossmab]]
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[[Category: Dp47]]
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[[Category: Fab fragment]]
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[[Category: Immune system]]
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[[Category: Vegf]]

Current revision

Variable heavy - variable light domain and Fab-arm CrossMabs with charged residue exchanges

6ghg, resolution 1.88Å

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