3b39

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|PDB= 3b39 |SIZE=350|CAPTION= <scene name='initialview01'>3b39</scene>, resolution 2.35&Aring;
|PDB= 3b39 |SIZE=350|CAPTION= <scene name='initialview01'>3b39</scene>, resolution 2.35&Aring;
|SITE=
|SITE=
-
|LIGAND=
+
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= dnaG, dnaP, parB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
|GENE= dnaG, dnaP, parB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
 +
|DOMAIN=
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|RELATEDENTRY=[[1dde|1DDE]], [[1dd9|1DD9]], [[1eqn|1EQN]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b39 OCA], [http://www.ebi.ac.uk/pdbsum/3b39 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b39 RCSB]</span>
}}
}}
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[[Category: zinc-finger]]
[[Category: zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:22:39 2008''

Revision as of 02:22, 31 March 2008

Image:3b39.jpg


PDB ID 3b39

Drag the structure with the mouse to rotate
, resolution 2.35Å
Ligands: , ,
Gene: dnaG, dnaP, parB (Escherichia coli)
Related: 1DDE, 1DD9, 1EQN


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Structure of the DnaG primase catalytic domain bound to ssDNA


Overview

Primases are essential RNA polymerases required for the initiation of DNA replication, lagging strand synthesis and replication restart. Many aspects of primase function remain unclear, including how the enzyme associates with a moving nucleic acid strand emanating from a helicase and orients primers for handoff to replisomal components. Using a new screening method to trap transient macromolecular interactions, we determined the structure of the Escherichia coli DnaG primase catalytic domain bound to single-stranded DNA. The structure reveals an unanticipated binding site that engages nucleic acid in two distinct configurations, indicating that it serves as a nonspecific capture and tracking locus for template DNA. Bioinformatic and biochemical analyses show that this evolutionarily constrained region enforces template polarity near the active site and is required for primase function. Together, our findings reverse previous proposals for primer-template orientation and reconcile disparate studies to re-evaluate replication fork organization.

About this Structure

3B39 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Identification of a DNA primase template tracking site redefines the geometry of primer synthesis., Corn JE, Pelton JG, Berger JM, Nat Struct Mol Biol. 2008 Feb;15(2):163-9. Epub 2008 Jan 13. PMID:18193061

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