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3k8p
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:3k8p.jpg|left|200px]] | ||
| - | < | + | ==Structural basis for vesicle tethering by the Dsl1 complex== |
| - | + | <StructureSection load='3k8p' size='340' side='right' caption='[[3k8p]], [[Resolution|resolution]] 2.60Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[3k8p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824] and [http://en.wikipedia.org/wiki/Atcc_56498 Atcc 56498]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K8P FirstGlance]. <br> | |
| - | + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |
| - | - | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">KLLA0C02695g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28985 ATCC 56498]), SEC39, DSL3, YLR440C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8p OCA], [http://pdbe.org/3k8p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3k8p RCSB], [http://www.ebi.ac.uk/pdbsum/3k8p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3k8p ProSAT]</span></td></tr> | |
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/SEC39_YEAST SEC39_YEAST]] Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.<ref>PMID:15242642</ref> <ref>PMID:15958492</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k8/3k8p_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k8p ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Vesicle trafficking requires membrane fusion, mediated by SNARE proteins, and upstream events that probably include "tethering," an initial long-range attachment between a vesicle and its target organelle. Among the factors proposed to mediate tethering are a set of multisubunit tethering complexes (MTCs). The Dsl1 complex, with only three subunits, is the simplest known MTC and is essential for the retrograde traffic of COPI-coated vesicles from the Golgi to the ER. To elucidate structural principles underlying MTC function, we have determined the structure of the Dsl1 complex, revealing a tower containing at its base the binding sites for two ER SNAREs and at its tip a flexible lasso for capturing vesicles. The Dsl1 complex binds to individual SNAREs via their N-terminal regulatory domains and also to assembled SNARE complexes; moreover, it is capable of accelerating SNARE complex assembly. Our results suggest that even the simplest MTC may be capable of orchestrating vesicle capture, uncoating, and fusion. | ||
| - | + | A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1.,Ren Y, Yip CK, Tripathi A, Huie D, Jeffrey PD, Walz T, Hughson FM Cell. 2009 Dec 11;139(6):1119-29. PMID:20005805<ref>PMID:20005805</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 3k8p" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Hughson, F M | + | __TOC__ |
| - | [[Category: Jeffrey, P D | + | </StructureSection> |
| - | [[Category: Ren, Y | + | [[Category: Atcc 18824]] |
| + | [[Category: Atcc 56498]] | ||
| + | [[Category: Hughson, F M]] | ||
| + | [[Category: Jeffrey, P D]] | ||
| + | [[Category: Ren, Y]] | ||
[[Category: Dsl1 complex]] | [[Category: Dsl1 complex]] | ||
[[Category: Endoplasmic reticulum]] | [[Category: Endoplasmic reticulum]] | ||
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[[Category: Snare protein]] | [[Category: Snare protein]] | ||
[[Category: Transport]] | [[Category: Transport]] | ||
| - | + | [[Category: Transport protein-transport protein complex]] | |
| - | + | ||
Current revision
Structural basis for vesicle tethering by the Dsl1 complex
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Categories: Atcc 18824 | Atcc 56498 | Hughson, F M | Jeffrey, P D | Ren, Y | Dsl1 complex | Endoplasmic reticulum | Er-golgi transport | Intracellular trafficking | Membrane | Multisubunit tethering complex | Protein transport | Snare protein | Transport | Transport protein-transport protein complex
