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3kvt

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|PDB= 3kvt |SIZE=350|CAPTION= <scene name='initialview01'>3kvt</scene>, resolution 2.00&Aring;
|PDB= 3kvt |SIZE=350|CAPTION= <scene name='initialview01'>3kvt</scene>, resolution 2.00&Aring;
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|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kvt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kvt OCA], [http://www.ebi.ac.uk/pdbsum/3kvt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3kvt RCSB]</span>
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[[Category: Pfaffinger, P J.]]
[[Category: Pfaffinger, P J.]]
[[Category: Shen, N V.]]
[[Category: Shen, N V.]]
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[[Category: ZN]]
 
[[Category: molecular recognition]]
[[Category: molecular recognition]]
[[Category: potassium channel]]
[[Category: potassium channel]]
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[[Category: zinc-binding]]
[[Category: zinc-binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:05:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:34:16 2008''

Revision as of 02:34, 31 March 2008


PDB ID 3kvt

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



TETRAMERIZATION DOMAIN FROM AKV3.1 (SHAW-SUBFAMILY) VOLTAGE-GATED POTASSIUM CHANNEL


Overview

The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.

About this Structure

3KVT is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.

Reference

Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290

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