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3l2p

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{{Seed}}
 
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[[Image:3l2p.jpg|left|200px]]
 
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<!--
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==Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States==
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The line below this paragraph, containing "STRUCTURE_3l2p", creates the "Structure Box" on the page.
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<StructureSection load='3l2p' size='340' side='right' caption='[[3l2p]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3l2p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3L2P FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
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-->
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LIG3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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{{STRUCTURE_3l2p| PDB=3l2p | SCENE= }}
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3l2p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3l2p OCA], [http://pdbe.org/3l2p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3l2p RCSB], [http://www.ebi.ac.uk/pdbsum/3l2p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3l2p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN]] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l2/3l2p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3l2p ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases DNA nick-joining and intermolecular DNA ligation. Yet, the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small angle x-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) form a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) -OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick-sensing and the transition from nick-sensing by the ZnF to nick-joining by the catalytic core. The collective results support a "jackknife model" whereby the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.
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===Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States===
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Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States.,Cotner-Gohara E, Kim IK, Hammel M, Tainer JA, Tomkinson AE, Ellenberger T Biochemistry. 2010 Jun 2. PMID:20518483<ref>PMID:20518483</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3l2p" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_20518483}}, adds the Publication Abstract to the page
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*[[DNA ligase|DNA ligase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 20518483 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20518483}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Human]]
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3L2P is a 4 chains structure with sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3L2P OCA].
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[[Category: Cotner-Gohara, E A]]
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[[Category: Ellenberger, T]]
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==Reference==
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[[Category: Hammel, M]]
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<ref group="xtra">PMID:20518483</ref><references group="xtra"/>
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[[Category: Kim, I K]]
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[[Category: Homo sapiens]]
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[[Category: Tainer, J A]]
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[[Category: Cotner-Gohara, E A.]]
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[[Category: Tomkinson, A]]
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[[Category: Ellenberger, T.]]
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[[Category: Hammel, M.]]
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[[Category: Kim, I K.]]
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[[Category: Tainer, J A.]]
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[[Category: Tomkinson, A.]]
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[[Category: Atp-binding]]
[[Category: Atp-binding]]
[[Category: Cell cycle]]
[[Category: Cell cycle]]
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[[Category: Phosphoprotein]]
[[Category: Phosphoprotein]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 14 16:19:28 2010''
 

Current revision

Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching Between Two DNA Bound States

3l2p, resolution 3.00Å

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