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6bbh

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Revision as of 07:48, 21 February 2019

The CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation

Structural highlights

6bbh is a 24 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	olf186-F, CRACM1, olf186, CG11430 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.^[1] ^[2]

Publication Abstract from PubMed

The store-operated calcium (Ca(2+)) channel Orai governs Ca(2+) influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca(2+) stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca(2+) selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation.

Structures reveal opening of the store-operated calcium channel Orai.,Hou X, Burstein SR, Long SB Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 2006 May 11;441(7090):179-85. Epub 2006 Apr 2. PMID:16582901 doi:http://dx.doi.org/nature04702
↑ Vig M, Peinelt C, Beck A, Koomoa DL, Rabah D, Koblan-Huberson M, Kraft S, Turner H, Fleig A, Penner R, Kinet JP. CRACM1 is a plasma membrane protein essential for store-operated Ca2+ entry. Science. 2006 May 26;312(5777):1220-3. Epub 2006 Apr 27. PMID:16645049 doi:http://dx.doi.org/10.1126/science.1127883
↑ Hou X, Burstein SR, Long SB. Structures reveal opening of the store-operated calcium channel Orai. Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233 doi:http://dx.doi.org/10.7554/eLife.36758

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6bbh"

@@ Line 3: / Line 3: @@
 <StructureSection load='6bbh' size='340' side='right' caption='[[6bbh]], [[Resolution|resolution]] 6.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6bbh]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BBH FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6bbh]] is a 24 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BBH FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbh OCA], [http://pdbe.org/6bbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bbh RCSB], [http://www.ebi.ac.uk/pdbsum/6bbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbh ProSAT]</span></td></tr>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">olf186-F, CRACM1, olf186, CG11430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbh OCA], [http://pdbe.org/6bbh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bbh RCSB], [http://www.ebi.ac.uk/pdbsum/6bbh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbh ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Drome]]
 [[Category: Burstein, S]]
 [[Category: Hou, X]]

6bbh

From Proteopedia

Revision as of 07:48, 21 February 2019

The CRAC channel Orai in an unlatched-closed conformation; K163W loss-of-function mutation

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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