Adenylosuccinate lyase
From Proteopedia
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| - | <StructureSection load=' | + | <StructureSection load='' size='450' side='right' caption='Adenylsuccinate lyase complex with AMP, oxalate, PEG 400 and Cl- ion (green) (PDB code [[2x75]])' scene='75/751814/Cv/1'> |
== Function == | == Function == | ||
| - | '''Adenylosuccinate lyase''' ( | + | '''Adenylosuccinate lyase''' (ASL) is a bifunctional enzyme acting in purine synthesis and purine nucleotide recycling<ref>PMID:10888601</ref>. ASL caalyzes two reactions: the cleavage of adenylsuccinate to AMP and fumarate and the cleavage of phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) into 5-aminoimidazole-4-carboxamide (AICAR) and fumarate. |
== Disease == | == Disease == | ||
| - | Mutations in | + | Mutations in ASL cause autosomal recessive disorder which manifests itself by encephalopathy with epilepsy and marked psychomotor retardation<ref>PMID:20054783</ref>. |
== Structural highlights == | == Structural highlights == | ||
| + | The ASL can be divided into 3 domains. The <scene name='75/751814/Cv/6'>active site</scene> is situated between the 3 domains and contains the product AMP and and oxalate<ref>PMID:20693687</ref>. Water molecules are shown as red spheres. <scene name='75/751814/Cv/7'>Oxalate binding</scene>. | ||
| - | + | ==3D structures of adenylosuccinate lyase== | |
| + | [[Adenylosuccinate lyase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
| - | ==3D structures of adenylosuccinate lyase== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | * Adenylosuccinate lyase | ||
| - | |||
| - | **[[5eyv]] – SmASL – ''Schistosoma mansoni''<br /> | ||
| - | **[[4nle]] – ASL – ''Mycobacterium smegmatis''<br /> | ||
| - | **[[4flc]] – hASL (mutant) – human<br /> | ||
| - | **[[5e3v]], [[4nsl]] – ASL – ''Salmonella typhimurium''<br /> | ||
| - | **[[3gzh]], [[2pts]] – EcASL – ''Escherichia coli''<br /> | ||
| - | **[[3bhg]] – ASL – ''Legionella pneumophila''<br /> | ||
| - | **[[2hvg]] – PvASL – ''Plasmodium vivax''<br /> | ||
| - | **[[1yis]] – ASL – ''Caernohabditis elegans''<br /> | ||
| - | **[[1dof]] – ASL – ''Pyrobaculum aerophilum''<br /> | ||
| - | **[[1f1o]] – ASL – ''Bacillus subtilis''<br /> | ||
| - | **[[1c3u]], [[1c3c]] – ASL – ''Termotoga maritima''<br /> | ||
| - | |||
| - | *Adenylosuccinate lyase binary complex | ||
| - | |||
| - | **[[5eyt]] – SmASL + AMP <br /> | ||
| - | **[[2j91]] – hASL + AMP <br /> | ||
| - | **[[4mx2]] – ASL + AMP – ''Leishmania donovani''<br /> | ||
| - | **[[4efc]] – ASL + AMP – ''Trypanosoma brucei''<br /> | ||
| - | **[[2qga]] – PvASL + AMP <br /> | ||
| - | **[[2ptr]] – EcASL (mutant) + substrate <br /> | ||
| - | **[[2pfm]] – ASL + malonate – ''Bacillus anthracis''<br /> | ||
| - | **[[5e3v]] – FtASL + fumarate - ''Francisella tularensis'' <br /> | ||
| - | |||
| - | *Adenylosuccinate lyase higher complexes | ||
| - | |||
| - | **[[2x75]] – ASL + AMP + oxalate – ''Staphylococcus aureus''<br /> | ||
| - | **[[4eei]] – FtASL + AMP + succinate <br /> | ||
| - | **[[2ptq]] – EcASL (mutant) + fumarate + AMP <br /> | ||
| - | **[[2vd6]] – hASL + fumarate + AMP + dicarboxymethyl-AMP<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
| + | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Kmoch S, Hartmannova H, Stiburkova B, Krijt J, Zikanova M, Sebesta I. Human adenylosuccinate lyase (ADSL), cloning and characterization of full-length cDNA and its isoform, gene structure and molecular basis for ADSL deficiency in six patients. Hum Mol Genet. 2000 Jun 12;9(10):1501-13. PMID:10888601
- ↑ Mierzewska H, Schmidt-Sidor B, Jurkiewicz E, Bogdanska A, Kusmierska K, Stepien T. Severe encephalopathy with brain atrophy and hypomyelination due to adenylosuccinate lyase deficiency--MRI, clinical, biochemical and neuropathological findings of Polish patients. Folia Neuropathol. 2009;47(4):314-20. PMID:20054783
- ↑ Fyfe PK, Dawson A, Hutchison MT, Cameron S, Hunter WN. Structure of Staphylococcus aureus adenylosuccinate lyase (PurB) and assessment of its potential as a target for structure-based inhibitor discovery. Acta Crystallogr D Biol Crystallogr. 2010 Aug;66(Pt 8):881-8. Epub 2010, Jul 9. PMID:20693687 doi:10.1107/S0907444910020081
