This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6a7f
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.== | ==The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.== | ||
| - | <StructureSection load='6a7f' size='340' side='right' caption='[[6a7f]], [[Resolution|resolution]] 3.40Å' scene=''> | + | <StructureSection load='6a7f' size='340' side='right'caption='[[6a7f]], [[Resolution|resolution]] 3.40Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br> | <table><tr><td colspan='2'>[[6a7f]] is a 30 chain structure with sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A7F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A7F FirstGlance]. <br> | ||
| Line 8: | Line 8: | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). | [[http://www.uniprot.org/uniprot/CAPSD_BPIKE CAPSD_BPIKE]] Self assembles to form a helical capsid wrapping up the viral genomic DNA. The capsid displays a filamentous structure with a length of 760-1950 nm and a width of 6-8 nm. The virion assembly and budding take place at the host inner membrane (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The filamentous bacteriophage IKe infects Escherichia coli cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 A. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a approximately 68-A-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 A and a twist of 38.52 degrees . The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, pi-pi interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained. | ||
| + | |||
| + | Cryo-electron microscopy structure of the filamentous bacteriophage IKe.,Xu J, Dayan N, Goldbourt A, Xiang Y Proc Natl Acad Sci U S A. 2019 Feb 28. pii: 1811929116. doi:, 10.1073/pnas.1811929116. PMID:30819888<ref>PMID:30819888</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6a7f" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Filamentous phage]] | [[Category: Filamentous phage]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Dayan, N]] | [[Category: Dayan, N]] | ||
[[Category: Goldbourt, A]] | [[Category: Goldbourt, A]] | ||
Revision as of 12:34, 13 March 2019
The cryo-EM structure of filamentous bacteriophage IKe major coat protein p8 shell assembly.
| |||||||||||
