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4czx

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Revision as of 09:00, 20 March 2019

Complex of Neurospora crassa PAN2 (WD40) with PAN3 (C-TERM)

Structural highlights

4czx is a 2 chain structure with sequence from Chrysonilia crassa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	4czv, 4czw, 4czy, 4d0k
Activity:	Poly(A)-specific ribonuclease, with EC number 3.1.13.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PAN2_NEUCR] Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein pabp-1, which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by xrn1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails (By similarity). [PAN3_NEUCR] Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex involved in cytoplasmic mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by polyadenylate-binding protein pab-1. Deadenylation of the 3'-tail to a length that is too short to bind pab-1 induces mRNA decay. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. pan-3 acts as a positive regulator for PAN activity, recruiting pan-2 to mRNA via its interaction with pab-1 (By similarity).

Publication Abstract from PubMed

The PAN2-PAN3 complex functions in general and microRNA-mediated mRNA deadenylation. However, mechanistic insight into PAN2 and its complex with the asymmetric PAN3 dimer is lacking. Here, we describe crystal structures that show that Neurospora crassa PAN2 comprises two independent structural units: a C-terminal catalytic unit and an N-terminal assembly unit that engages in a bipartite interaction with PAN3 dimers. The catalytic unit contains the exonuclease domain in an intimate complex with a potentially modulatory ubiquitin-protease-like domain. The assembly unit contains a WD40 propeller connected to an adaptable linker. The propeller contacts the PAN3 C-terminal domain, whereas the linker reinforces the asymmetry of the PAN3 dimer and prevents the recruitment of a second PAN2 molecule. Functional data indicate an essential role for PAN3 in coordinating PAN2-mediated deadenylation with subsequent steps in mRNA decay, which lead to complete mRNA degradation.

An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.,Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, Weichenrieder O, Izaurralde E Nat Struct Mol Biol. 2014 Jun 1. doi: 10.1038/nsmb.2837. PMID:24880343^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, Weichenrieder O, Izaurralde E. An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay. Nat Struct Mol Biol. 2014 Jun 1. doi: 10.1038/nsmb.2837. PMID:24880343 doi:http://dx.doi.org/10.1038/nsmb.2837

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4czx"

@@ Line 1: / Line 1: @@
 ==Complex of Neurospora crassa PAN2 (WD40) with PAN3 (C-TERM)==
-<StructureSection load='4czx' size='340' side='right' caption='[[4czx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+<StructureSection load='4czx' size='340' side='right'caption='[[4czx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4czx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CZX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4czx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CZX FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4czv|4czv]], [[4czw|4czw]], [[4czy|4czy]], [[4d0k|4d0k]]</td></tr>
@@ Line 20: / Line 20: @@
 </div>
 <div class="pdbe-citations 4czx" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Ribonuclease|Ribonuclease]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Chrysonilia crassa]]
+[[Category: Large Structures]]
 [[Category: Izaurralde, E]]
 [[Category: Jonas, S]]

4czx

From Proteopedia

Revision as of 09:00, 20 March 2019

Complex of Neurospora crassa PAN2 (WD40) with PAN3 (C-TERM)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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