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| - | [[Image:1mc4.png|left|200px|thumb|Crystal Structure of Aspartate-semialdehyde dehydrogenase, [[1mc4]]]]
| + | <StructureSection load='1mb4' size='350' side='right' caption='Aspartate-semialdehyde dehydrogenase complex with NADP and substrate analog (PDB code [[1mb4]])' scene='45/452498/Cv/2'> |
| - | {{STRUCTURE_1mc4| PDB=1mc4 | SIZE=400| SCENE=Aspartate-semialdehyde_dehydrogenase/Cv/1 |right|CAPTION=Aspartate-semialdehyde dehydrogenase, [[1mc4]] }}
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| - | [[Aspartate-semialdehyde dehydrogenase]] (ASADH) is an enzyme which is part of the biosynthesis of amino acids. It catalyzes the conversion of L-aspartate 4-semialdehyde (ASA) + phosphate + NADP to L-4-aspartyl phosphate + NADPH + H+. The images at the left and at the right correspond to one representative ASADH, ''i.e.'' the crystal structure of Aspartate-semialdehyde dehydrogenase from ''Vibrio cholerae'' ([[1mc4]]).
| + | == Function == |
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| - | {{TOC limit|limit=2}}
| + | '''Aspartate-semialdehyde dehydrogenase''' (ASADH) is an enzyme which is part of the biosynthesis of amino acids in bacteria, plants and fungi. It catalyzes the conversion of L-aspartate 4-semialdehyde (ASA) + phosphate + NADP to L-4-aspartyl phosphate + NADPH + H+. |
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| - | == 3D Structures of Aspartate-semialdehyde dehydrogenase == | + | == Structural highlights == |
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| - | [[2qz9]], [[1mc4]] – VcASADH II – ''Vibrio cholerae''<br />
| + | ASADH contains 2 domains. The N terminal domain contains the <scene name='45/452498/Cv/7'>active site</scene> and the <scene name='45/452498/Cv/9'>NADP-binding site</scene>. The active site contains a <scene name='45/452498/Cv/10'>cysteine residue</scene> (C134 in ''Vibrio Cholerae'') which binds to inhibitors. The C terminal contains the homodimer intersubunit contacts. <ref>PMID:12493825</ref> |
| - | [[2yv3]] – ASADH – ''Thermus thermiphilus''<br />
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| - | [[1t4d]], [[1t4b]], [[1brm]] - EcASADH β - ''Escherichia coli''<br />
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| - | [[1pu2]], [[1oza]] - HiASADH (mutant) – ''Haemophilus influenzae''<br />
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| - | [[1nwc]] – HiASADH<br />
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| - | [[1nwh]] – HiASADH reaction intermediate<br />
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| - | ===ASADH binary complex=== | + | == 3D Structures of Aspartate-semialdehyde dehydrogenase == |
| - | | + | [[Aspartate-semialdehyde dehydrogenase 3D structures]] |
| - | [[2r00]] - VcASADH II + ASA<br /> | + | |
| - | [[3llg]] – ASADH + glycerot + Pi – ''Mycobacterium tuberculosis''<br />
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| - | [[3hsk]] – ASADH + NADP – ''Candida albicans''<br />
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| - | [[1ys4]] - ASADH + NADP – ''Methanocaldococcus jannaschii''<br />
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| - | [[1nx6]] - HiASADH reaction intermediate +Pi<br />
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| - | [[1ta4]] - HiASADH + As<br />
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| - | [[1tb4]] - HiASADH + IO4<br />
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| - | [[1q2x]] - HiASADH (mutant) + ASA<br />
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| - | ===ASADH ternary complex===
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| - | [[1pqu]] - HiASADH (mutant) + NADP + cacodylate + substrate analog<br />
| + | </StructureSection> |
| - | [[1pqp]] - HiASADH (mutant) + ASA + Pi<br />
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| - | [[1gl3]] - EcASADH β + NADP + substrate analog<br />
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| - | [[1mb4]] - VcASADH + NADP + substrate analog<br />
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| | + | == References == |
| | + | <references/> |
| | [[Category:Topic Page]] | | [[Category:Topic Page]] |
